摘要
为深入了解麝鼠生殖腺发育的规律,以及探究这些凋亡体相关基因在麝鼠生殖腺发育周期中的作用,试验采用RT-qPCR技术检测了成年雄性麝鼠繁殖期与非繁殖期前列腺、精囊腺和睾丸中组成凋亡体3个基因的mRNA表达水平,并通过免疫印迹技术验证部分结果。结果表明:Cytc基因、Caspase-9基因和Apaf-1基因在非繁殖期的表达量均高于繁殖期,且差异显著(P≤0.05)。同时,Cytc基因和Apaf-1基因在前列腺和精囊腺两个组织中,繁殖期与非繁殖期两个时期的mRNA表达水平变化接近,而睾丸中的m RNA表达水平变化远高于二者。Caspase9基因在睾丸和精囊腺中,繁殖期与非繁殖期两个时期的mRNA表达水平变化接近,远高于前列腺中的m RNA表达水平变化。说明凋亡体3个基因在繁殖结束后前列腺、精囊腺和睾丸的萎缩过程中起到重要的调控作用。
In order to understand the development of muskrat gonads and explore the role of these apoptotic genes in the development cycle of muskrat gonads,RT-qPCR was used to detect the mRNA expression levels of three apoptotic genes in the prostate gland,seminal vesicle and testis of adult male muskrats at reproductive and non-reproductive stages,and western blot was used to verify part of the results.The results showed that the expression levels of Cytc gene,Caspase-9 gene and Apaf-1 gene in non-reprodctive period were higher than that in reprodctive period,and the differences were significant(P≤0.05).Meanwhile,the mRNA expression levels of Cytc and Apaf-1 in prostate and seminal vesicle tissues were similar in reproductive and non-reproductive period,while the mRNA expression levels in testis were much higher than those in the two tissues.The changes of m RNA expression levels of Caspase9 gene in testis and seminal vesicle during reproductive and non-reproductive periods were close to each other,which was much higher than that in prostate.These results indicated that the three apoptotic genes played an important role in the atrophy of prostate,seminal vesicle and testis after reproduction.
作者
李天峰
叶筱彤
雷行
满欣
白素英
LI Tianfeng;YE Xiaotong;LEI Xing;MAN Xin;BAI Suying(College of Wildlife and Protected Area,Northeast Forestry University,Harbin 150040,China;National Forestry and Grassland Administration Detecting Center of Wildlife,Harbin 150040,China)
出处
《黑龙江动物繁殖》
2021年第3期9-15,共7页
Heilongjiang journal of animal reproduction
关键词
麝鼠
生殖腺
凋亡体
繁殖
muskrat
gonad
apoptosis body
reproduction
作者简介
李天峰(1996-),男(蒙古族),硕士研究生,研究方向为动物遗传育种与繁殖,392312416@qq.com;通信作者:白素英(1969-),女,副教授,博士,硕士生导师,研究方向为动物遗传育种与繁殖,baisynefu@qq.com.
