摘要
目的探讨TRIP13在肺癌中的作用和信号传导机制。方法通过基因转染和siRNA干扰调控肺癌细胞中TRIP13的表达量;采用克隆形成实验、划痕实验以及基质胶侵袭实验观察TRIP13对肺癌细胞增殖、迁移和侵袭能力的影响;采用Western blot检测TRIP13的表达量对Hippo信号通路蛋白的影响。结果检索GEPIA数据库发现,TRIP13在肺癌组织中的表达水平均明显高于癌旁正常肺组织(P<0.01);TRIP13的高表达与肺癌患者的低生存率正相关(P=0.0011)。TRIP13的过表达可显著促进肺癌细胞的增殖、迁移和侵袭能力,并显著上调YAP、RhoA、CyclinB1和MMP9的表达,抑制LATS、MST和p-YAP的表达(P<0.05);而下调TRIP13后,则逆转上述结果。结论TRIP13调控Hippo信号通路促进非小细胞肺癌的增殖、迁移和侵袭。
Objective To investigate the role and molecular mechanism of TRIP13 in lung cancer cells.Methods The expression of TRIP13 in lung cancer cells was regulated by gene transfection or siRNA interference.The effects of TRIP13 on the proliferation,migration and invasion of lung cancer cells were examined using clone formation assay,scratch assay,matrigel invasion assay.The levels of proteins in Hippo signaling pathway were examined by Western blot.Results The expression of TRIP13 was significantly higher in lung cancer tissues than in normal lung tissues based on the GEPIA database(P<0.01),and the high expression of TRIP13 was correlated with poor prognosis(P=0.0011).Overexpression of TRIP13 significantly promoted the proliferation and migration and invasion of lung cancer cells.TRIP13 overexpression significantly upregulated the expression of YAP and RhoA and Cyclin B1 as well as MMP9,but inhibited the expression of LATS,MST and p-YAP(P<0.05).After decreasing the expression of TRIP13,the above results were reversed.Conclusion TRIP13 promotes the proliferation,migration and invasion of NSCLCs via Hippo signaling pathway.
作者
李志瀚
雷蕾
郑艺雯
黄文镜
刘晨晨
徐洪涛
LI Zhi-han;LEI Lei;ZHENG Yi-wen;HUANG Wen-jing;LIU Chen-chen;XU Hong-tao(Department of Pathology,the First Hospital,the College of Basic Medical Science,China Medical University,Shenyang 110001,China)
出处
《解剖科学进展》
CAS
2022年第4期487-490,494,共5页
Progress of Anatomical Sciences
基金
辽宁省高等学校优秀人才支持计划(LR2015067)
关键词
TRIP13
Hippo信号通路
肺癌
增殖
侵袭
Thyroid hormone receptor interactor 13
Hippo signaling pathway
lung cancer
proliferation
invasion
作者简介
通信作者:徐洪涛
