摘要
1.用母猫肠系膜内的环层小体,Carnoy固定液固定,石蜡包埋,切成10微米。2.用Feulgen染色,板层内的细胞核有阳性反应,用甲绿——哌啷咛染色,核呈蓝紫色,且均与附近的成纤维细胞核有相同的构造。3.用过氯酸在70℃下水解3小时後再用上法染色,就不再见有这些核的出现,而用过氯酸在冰箱中(1.5—2℃)过夜,核的颜色和构造都与对照片同。4.用蒸馏水代替过氯酸在70℃或1.5—2℃同样处理後染色也和对照片同。5.由以上的结果,我们认为环层小体的板层内有核的存在。根据我们的观察核在环层本身的两层膜内。
Pacinian corpuscles from cat mesentery were fixed in Carnoy’s fixative,paraffinsectioned and stained in Feulgen reagent and methyl green-pyronin.Controlled sectionswere hydrolyzed in 20%perchloric acid at 70℃and 10%perchloric acid at 1-4℃.Itwas found that the so-called nuclei in the lamellae were Feulgen positive and stainedbluish purple after the methyl green-pyronin method.They disappeared after perchloric acidhydrolysis in 70℃and showed no change after cold treatment.Their histochemicalreactions,stainability and detailed structures were similar to the nuclei of the connectivecells next to the corpuscle.It was therefore concluded the nuclear like bodies in the Paciniancorpuscles studied were true nuclei as mentioned in histological textbooks and not merely thecondensation of fibrils as W.Schwarz recently claimed after electron microscopic observation.These nuclei were located in between the fibrils of the lamellae.
作者
赵宗昌
CHAO CHUNG-CHANG(Department of Anatomy,Chinese Union Medical College,Peking)
出处
《解剖学报》
CAS
1954年第2期179-184,284,共7页
Acta Anatomica Sinica
作者简介
赵宗昌,最近通信處为四川重庆小龙坎2102号信箱
