摘要
取成年兔46只,分成二组:实验组42只,分别注HRP于胸_(11,12)、腰_(1,2)和骶_(2~4)节段各6例;对照组4只,分别注HRP于耳静脉和脑脊液各2例。然后在丘脑下部观察逆行标记细胞和顺行标记神经末梢分支(终枝)。作者在实验动物的本文用家兔九只。五只按Sawyer氏法定位,沿APO平面作额面连续切片,另四只剥离大脑皮质,暴露海马,作海马部位的连续切片。交替用尼氏法和Glees法镀银染色,结果如下:1.海马:长度为28~36mm,宽6~8.5mm,厚2.68~3.39mm。海马内锥体细胞总数平均为7.36×106个,颗粒细胞总数为4.93×106个,篮细胞总数为2.40×105个。锥体细胞:颗粒细胞:篮细胞=30:20:1。CA1区锥体细胞总数为4.11×106个,CA3区锥体细胞总数为2.48×106个;CA1:CA3=1.66:1。从胞体面积及胞核面积的统计学分析看,二者在形态上有一定差异。海马内结构的另一特点是锥体细胞之主干树突与齿状回的颗粒细胞排列紧密有序,类似阵列结构。2.单侧穹窿柱(FN)的神经纤维总数平均为83,700根,单侧乳头丘脑束(MT)的神经纤维总数为58,740根;MT:FN=1:1.42。3.乳头体核组细胞总数为113,500个,丘脑前核组为165,600个;乳头体核组:丘脑前核组=1:1.46。结合以上结果,对海马结构特点的机能意义进行了讨论。丘脑下部内侧基底部(medial basal hypothalimus MBH)、腹内侧核、室旁核、背侧区、外侧区、后核、穹窿周核和视上核内见到标记细胞。新发现的终支主要分布于室旁核背外侧缘、背侧区和外侧区背侧,偶见于后核和MBH中。本文证实丘脑下部有大量神经元直接投射到支配盆腔脏器的所有脊髓节段,且这些节段有上行纤维直接投射到丘脑下部。所以,丘脑下部与支配盆腔脏器脊髓节段间有往返直接投射。对照组未见标记细胞和终支。
46 rabbits were divided into two groups.HRP was injected into one of the 7 segments of spinal cord(T_(11,12)、L_(1,2)、S_(2~4))unilaterally in 42 adult rabbits in the experimental group.HRP was also injected into the vein of the ear(2 cases)and cerebrospinal fluid(2 cases)as the control group.The hypothalamus were cut at 40μm.The sections were incubated with BDHC and OD by Mesulam's 8th and Edward's procedures.Labeled cell were found in the medial basal hypothalamus(MBH),ventromedial nucleus(VM),paraventricular nucleus(PV),dorsomedial nucleus(DM),posterior nucleus(PN),supraoptic nucleus(SN),lateral nucleus(LN),anterior nucleus(AN),perifornical nucleus and zona incerta in all cases.The PV was most heavily labeled.The LN,PN and DM were less so.Some labeled cells were found in the MBH,VM and AN.A few labeled cells were present in the SN.Labeled terminal and preterminal axons were found in the dorsolateral margin of the PV and in the LN、PN and MBH.Labeled terminals of the hypothalamus,which were discovered in this study,has not mentioned in the literature available to us.This study demonstrated reciprocal projection between the hypothalamus and the segments of the spinal cord innervating pelvic organs.
作者
钟昌奇
李珍年
Zhong Changqi;Li Zhennian(China Medical College,Shenyang)
出处
《解剖学报》
CAS
1985年第3期285-291,343,共8页
Acta Anatomica Sinica
关键词
丘脑下部
脊髓
往返联系
HRP法
家兔
Hypothalamus
Spinal
Reciprocal connections
HPR method
Rabbit
