摘要
应用胶原酶与链霉蛋白酶消化和分解破坏大鼠肝细胞的方法,获得非肝细胞;再经贴壁培养,成功地获得大量高纯度的枯否细胞。枯否细胞获得率为8.3×10^(6)个/每克肝组织,胞质内均含有在体吞噬的墨汁颗粒。培养成活的枯否细胞呈典型巨噬细胞形态,电镜观察下表而有发达的伪足和微绒毛等结构,胞质内含大量溶酶体。以绵羊红细胞(SRBC)检测枯否细胞免疫活性,90%以上的细胞在特异抗体介导下形成SRBC花环,并能活跃吞噬SRBC。电镜观察了枯否细胞花环和吞噬功能。本实验国内首次分离培养成功大鼠肝枯否细胞,并证明具有良好的免疫活性,这对进一步研究枯否细胞的功能,尤其是它对肿瘤的免疫监视作用有实际意义。
The viable nonparcnchymal cells(NPC)of rat livers were prepared by collagenase perfusion and pronasc treatment.Following cell culture of NPC,the plenty of Kupffer cells(KC)had been purificd by attachment.The yield of KC is 8.3×10^(6) cells/g liver.The KC exibited typical macrophage characteristics under phasecontrast microscope,TEM,and SEM.By means of immunological method,it was identified that more than 90%of KC formed rosettes with opsonized SRBC and engulfed several SRBCs.The rosettes and phagocytosis of KC had also been observed with TEM and SEM.The results show that the isolation method of KC mentioned above is more cinvenient and the isolated KC keep perfect immune activities.
作者
韩伟
成令忠
顾云娣
Han Wei;Cheng Linzhong;Gu Yundi(Department of Histology and Embryology,Shanghai Medical University)
出处
《解剖学杂志》
CAS
1988年第2期71-74,143,共5页
Chinese Journal of Anatomy