摘要
本文以黑木耳醇提物和水提物为研究对象,以对胰脂肪酶活性的抑制率为指标,分别对其提取工艺进行了单因素和正交试验,选取优化后抑制率高的提取物进行抑制类型和3T3-L1前脂肪细胞方面的研究。结果表明醇提物的最佳提取工艺为提取温度70℃、提取时间1h、乙醇浓度90%、料液比1:20,抑制胰脂肪酶的IC50=681.56μg/mL。水提物的最佳提取工艺为提取温度70℃、提取时间2h、料液比1:40,抑制胰脂肪酶的IC50=850.59μg/mL,醇提物的抑制效果优于水提物。醇提物对胰脂肪酶的抑制类型为非竞争性抑制,抑制常数为4.69mg/mL;醇提物浓度低于1mg/mL时,对3T3-L1前脂肪细胞的活性无影响;浓度高于400μg/mL时即可显著抑制前脂肪细胞的分化。
Using inhibition rate of pancreatic lipase activity as index,the extracting process of alcohol extract and water extract of Auricularia heimuer fruiting body(AHFB)were optimized by single factor and orthogonal experiments,respectively.The extract with the highest inhibition rate was used to determine inhibition types and inhibition effects on 3 T3-L1 preadipocytes.The results showed that the optimum extracting conditions of ethanol extract of AHFB included the extraction temperature of 70°C,extraction duration of 1 h,ethanol concentration of 90%,and solid-liquid ratio of 1:20.IC50 of the ethanol extract inhibiting pancreatic lipase activity obtained was 681.56μg/mL.The optimum extraction conditions of water extract of AHFB were the extraction temperature of 70°C,extraction duration of 2 h and solid material-liquid ratio of 1:40.IC50 of the water extract inhibiting pancreatic lipase activity obtained was 850.59μg/mL.The inhibiting rate of ethanol extract on pancreatic lipase activity was higher than that of water extract.The inhibition of ethanol extract on pancreatic lipase was non-competitive,and the inhibition constant was 4.69 mg/mL.The viability of 3 T3-L1 preadipocytes was not affected when the concentration of ethanol extract was lower than 1 mg/mL.The concentration over 400μg/mL could significantly inhibit the differentiation of 3 T3-L1 preadipocytes.
作者
黄琳翔
施乐乐
蔡志英
纪鹏伟
王陈荔
陈炳智
江玉姬
谢宝贵
HUANG Lin‐Xiang;SHI Le‐Le;CAI Zhi‐Ying;JI Peng‐Wei;WANG Chen‐Li;CHEN Bing‐Zhi;JIANG Yu‐Ji;XIE Bao‐Gui(ollege of Food Sciences,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Fujian Edible Fungi Technical Popularized Station,Fuzhou,Fujian 350003,China;Agriculture Bureau of Longhai City,Longhai,Fujian 363101,China;Xinfa Biotechnology Co.Ltd.,Zhangzhou,Fujian 363107,China;Mycological Research Center,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第2期441-451,共11页
Mycosystema
基金
福建农林大学科技创新基金(CXZX2017410).
作者简介
Corresponding authors:江玉姬,E‐mail:jyj1209@163.com;Corresponding authors:陈炳智,E‐mail:cbz_2006@163.com
