摘要
目的探讨壳寡糖(COS)灌胃治疗急性放射性肠炎(ARE)的可行性。方法选取6只普通级健康比格犬为研究对象。经放射线腹部靶区照射建立比格犬ARE模型。观察各实验犬照射第3天的症状。分别于照射前、照射后、治疗后,每天于固定时间观察并记录比格犬的体温、体质量。分别于照射前、照射第3天、治疗第8天,收集并比较所有实验犬白细胞总数、中性粒细胞总数、C反应蛋白(CRP)。分别于照射第3天、治疗第8天,对所有实验犬进行肠镜检查,收集内镜下图片,并进行维也纳直肠镜评分。结果所有实验犬照射第3 d均出现明显RE症状,均达到2度放射性肠损伤表现,包括摄入减少、恶心、呕吐、腹泻、间断便血等。COS灌胃治疗第8天,所有实验犬体质量、体温平均值基本恢复到照射前水平。照射第3天,各实验犬的白细胞总数、中性粒细胞总数、CRP均高于照射前,差异均有统计学意义(P<0.05);治疗第8天,各实验犬的白细胞总数、中性粒细胞总数均低于照射第3天,差异均有统计学意义(P<0.05)。各实验犬治疗第8天的维也纳直肠镜评分为(1.33±0.52)分,显著低于照射第3天的(6.83±0.75)分,差异有统计学意义(P<0.05)。结论应用COS溶液灌胃对改善ARE实验犬的临床症状、炎症指标、肠镜下黏膜炎症均有效,初步证实了COS使用的安全性,为进一步开展机制研究奠定一定基础。
Objective To investigate the feasibility of chitooligosaccharide(COS)intragastric treatment for acute radiation enteritis(ARE).Methods Six normal healthy beagles were selected as the research objects.The ARE model of beagle dog was established by abdominal target irradiation.The symptoms of dogs were observed on the 3 rd day of irradiation.The body temperature and body weight of beagles were observed and recorded at a fixed time every day before,after and after irradiation.The total number of white blood cells,neutrophils,and C-reactive protein(CRP)were collected and compared before irradiation,on the 3 rd day of irradiation,and on the 8 th day of treatment.On the 3 rd day of irradiation and 8 th day of treatment,colonoscopy was performed on all experimental dogs,and the endoscopic images were collected and the Vienna colonoscopy score was performed.Results On the 3 rd day of irradiation,all dogs showed obvious symptoms of RE,which reached grade 2 radiation-induced intestinal injury,including decreased intake,nausea,vomiting,diarrhea,intermittent hematochezia,etc.On the 8 th day of COS gavage treatment,the average body weight and body temperature of all experimental dogs basically recovered to the level before irradiation.On the third day of irradiation,the total white blood cell count,neutrophil count and CRP of all experimental dogs were higher than those before irradiation,and the differences were statistically significant(P<0.05).On the 8 th day of treatment,the total number of white blood cells and neutrophils in all experimental dogs were lower than those on the 3 rd day of irradiation,and the differences were statistically significant(P<0.05).The score of Vienna colonoscopy on the 8 th day of treatment was(1.33±0.52),which was significantly lower than that on the 3 rd day of irradiation(6.83±0.75),and the difference was statistically significant(P<0.05).Conclusion The application of COS solution gavage is effective in improving clinical symptoms,inflammatory indicators and mucosal inflammation under colonoscopy in ARE experimental dogs,which preliminarily confirms the safety of COS use and lays a certain foundation for further mechanism research.
作者
罗娅媛
白燕鸥
吕伟彦
冯吉
李宏宇
郭晓钟
刘旭
LUO Ya-yuan;BAI Yan-ou;LYU Wei-yan;FENG Ji;LI Hong-yu;GUO Xiao-zhong;LIU Xu(Department of Gastroenterology,General Hospital of Northern Theater Command,Shenyang 110016,China)
出处
《临床军医杂志》
CAS
2022年第9期930-933,937,共5页
Clinical Journal of Medical Officers
基金
军队后勤科研面上项目(CLB20J037)
关键词
急性放射性肠炎
壳寡糖
灌胃
疗效
Acute radiation enteritis
Chitooligosaccharide
Gavage
Curative effect
作者简介
第一作者:罗娅媛(1994-),女,河南人,医师,硕士;通信作者:刘旭,E-mail:we1980@163.com
