摘要
20(S)-原人参二醇作为研究对象,用反溶剂沉淀法制备20(S)-原人参二醇纳米混悬液。通过优化液相的条件,本章建立了原人参二醇HPLC的体外分析方法,确定了乙腈∶水(v/v)=90∶10(v/v)作为流动相,波长210nm的液相条件。对标准曲线,精密度和准确度的考察结果显示:原人参二醇在23.08~1154μg/mL范围内线性良好,日内、日间精密度RSD值小于3%,准确度在96%~103%之间,加样回收率为100.84%,该方法满足体外检测原人参二醇定量的要求。考察冻干保护剂,最后确定为1.5%的羟丙基-β-环糊精。
Using 20(S)-protopanaxadiol as research object,20(S)-protopanaxadiol nanosuspension was prepared by anti-solvent precipitation.An in vitro analytical method of protopanaxadiol by HPLC was established,and the liquid phase conditions were optimized as follows:the mobile phase acetonitrile and water(vacetonitrile∶vwater=90∶10),the wavelength 210 nm.The standard curve,precision and accuracy were investigated.The results showed that the protopanaxadiol had good linearity in the range of 23.08~1154μg/mL,intra-day and inter-day precision RSD values were less than 3%,the accuracy was between 96%~103%,and the recovery rate was 100.84%.This method met the requirements for the in vitro determination of protopanaxadiol.At last,its lyophilized protective agents were screened,and 1.5%hydroxypropyl-β-cyclodextrin was finally determined as suitable lyophilized protective agent.
作者
陈晨
孙倩
CHEN Chen;SUN Qian(Liaoning Vocational College of Medicine,Liaoning Shenyang 113001,China)
出处
《辽宁化工》
CAS
2020年第2期140-143,共4页
Liaoning Chemical Industry
关键词
原人参二醇
含量测定
冻干保护剂
protopanaxadiol
content determination
lyophilized protective agent
作者简介
陈晨(1990-),女,助教,硕士,辽宁省沈阳市人,2016年毕业于澳门大学中药学专业,研究方向:药物分析;通讯作者:孙倩(1985-),女,讲师,硕士,研究方向:药物分析。
