摘要
目的:探讨益气强心煎剂对慢性心力衰竭大鼠血清AngⅡ浓度以及心肌组织periostin、collageⅠ蛋白表达的影响。方法:成年雄性SD大鼠120只,随机选取20只作为对照组,余下100只建立心衰模型。造模成功后,随机分成5组,模型组(每日1次灌服同等体积的生理盐水)、益气强心煎剂高剂量组(以6.36 g/100 g体质量灌胃)、益气强心煎剂中剂量组(以3.18 g/100 g体质量灌胃)、益气强心煎剂低剂量组(以1.59 g/100 g体质量灌胃)、西药对照组(以洛丁新0.45 mg/kg体质量灌胃),上述药物与生理盐水制成3 mL溶液或混悬液,每日灌胃1次。用药4周后,观察所有大鼠心脏形态学、血清AngⅡ浓度以及心肌组织periostin、collageⅠ蛋白表达水平。结果:心脏形态学方面,对照组心肌细胞排列整齐紧密,分布均匀,形态正常。模型组心肌细胞大面积变性坏死,分布不均,间质纤维组织增生,少量炎性细胞浸润,心肌细胞交织成网状,心肌纤维出现断裂缺失、坏死和纤维化改变。AngⅡ浓度方面,与模型组比较,益气强心汤高剂量组、洛丁新组大鼠血清AngⅡ浓度偏低(P<0.05),与益气强心汤高剂量组相比,洛丁新组AngⅡ浓度较低(P<0.01)。与模型组比较,各组大鼠心肌组织periostin蛋白表达水平较低,其中洛丁新组>益气强心汤高剂量组>益气强心汤低、中剂量组(P<0.01);与模型组比较,益气强心汤中、高剂量组,洛丁新组大鼠心肌组织collageⅠ蛋白表达水平较低,其中洛丁新组>益气强心汤高剂量组>益气强心汤低剂量组(P<0.01)。结论:益气强心汤能够有效调节慢性心力衰竭大鼠心肌纤维化发展,延缓左室重构,改善心功能,其机制可能与其调节慢性心衰大鼠血清AngⅡ浓度,抑制collagenⅠ与periostin蛋白表达相关。
Objective:To investigate the effects of Yiqi Qiangxin decoction on serum AngⅡconcentration and expression of myocardial tissue periostin and collagen I protein in rats with chronic heart failure.Methods:120 adult male Sprague-Dawley rats,twenty of them were randomly selected as the control group,and the remaining 100 were used to establish the heart failure model.After successful modeling,they were randomly divided into 5 groups,model group(administered the same volume of normal saline once a day),Yiqi Qiangxin decoction high-dose group(administered at 6.36 g/100 g body weight),medium-dose group(administered at 3.18 g/100 g body weight),low-dose group(administered at 1.59 g/100 g body weight),western medicine control group(administered with Luodingxin 0.45 mg/kg body weight),the above drugs and physiological saline made 3 mL solution or suspension,once a day.After 4 weeks of treatment,the morphology of the heart,the serum AngⅡconcentration and the protein expression levels of myocardial tissue periostin and collagen I were observed.Results:In terms of cardiac morphology,the cardiomyocytes of the control group were arranged neatly and closely,with uniform distribution and normal morphology.The model group myocardial cells had large area degeneration and necrosis,uneven distribution,interstitial fibrous tissue hyperplasia,a small amount of inflammatory cell infiltration,myocardial cells intertwined into a network,myocardial fibers showed loss of rupture,necrosis and fibrosis.In terms of AngII concentration,compared with the model group,the high dose group and the Luodingxin group was lower(P<0.05),compared with the high dose group,the concentration of AngⅡin Luodingxin group was lower(P<0.01).Compared with the model group,the expression level of periostin protein in myocardial tissue of each group was lower.From high to low,followed by Luodingxin group,high dose group,low and medium dose groups(P<0.01).Compared with the model group,the expression of collagenⅠprotein in the myocardial tissue of rats in medium and high dose group and Luodingxin group was low,from high to low,followed by Luodingxin group,high dose group,low dose group(P<0.01).Conclusion:Yiqi Qiangxin Decoction could effectively regulate the development of myocardial fibrosis in rats with chronic heart failure,delay left ventricular remodeling and improve cardiac function.The mechanism might be related to regulate the serum AngⅡconcentration in rats with chronic heart failure and inhibite the protein expression of collagenⅠand periostin.
作者
宋筱靓
王帅
邹晓明
王凤荣
SONG Xiaoliang;WANG Shuai;ZOU Xiaoming;WANG Fengrong(Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,Liaoning,China)
出处
《辽宁中医药大学学报》
CAS
2019年第11期44-48,共5页
Journal of Liaoning University of Traditional Chinese Medicine
基金
辽宁省科技计划项目(2012225103).
关键词
心力衰竭
益气强心煎剂
心室重构
heart failure
Yiqi Qiangxin decoction
ventricular remodeling
作者简介
宋筱靓(1982-),男,辽宁沈阳人,副主任医师,硕士,研究方向:中西医结合防治老年病;通讯作者:王凤荣(1962-),女,辽宁沈阳人,主任医师,博士研究生导师,博士,研究方向:中西医结合防治心血管疾病。E-mail:wfr925@126.com。
