摘要
[Objectives]This study aimed to establish the fingerprint of Cryptolepis buchananii Roem.et Schult.by HPLC.[Methods]Taking the peak of chlorogenic acid as the references,the 10 batches of C.buchananii Roem.et Schult.materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5μm,4.6 mm×250 mm),detection wavelength of 323 nm,column temperature of 25℃,mobile phase of methanol-0.1%phosphoric acid solution,gradient elution and injection volume of 20μL.The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A).[Results]Among the HPLC fingerprints of the 10 batches of C.buchananii Roem.et Schult.materials,there are 13 common peaks,and their similarity is all greater than 0.9,indicating good similarity.[Conclusions]This study can provide a scientific basis for the quality evaluation of C.buchananii Roem.et Schult.
[Objectives] This study aimed to establish the fingerprint of Cryptolepis buchananii Roem. et Schult. by HPLC. [Methods] Taking the peak of chlorogenic acid as the references, the 10 batches of C. buchananii Roem. et Schult. materials were detected by HPLC under the conditions of column of Thermo ODS-2 Hypersil(5 μm, 4.6 mm×250 mm), detection wavelength of 323 nm, column temperature of 25 ℃, mobile phase of methanol-0.1% phosphoric acid solution, gradient elution and injection volume of 20 μL. The common peak identification and similarity evaluation were carried out according to Similarity Evaluation System for Chromatographic Fingerprint of Chinese Traditional Medicine(Version 2004 A). [Results] Among the HPLC fingerprints of the 10 batches of C. buchananii Roem. et Schult. materials, there are 13 common peaks, and their similarity is all greater than 0.9, indicating good similarity. [Conclusions] This study can provide a scientific basis for the quality evaluation of C. buchananii Roem. et Schult.
基金
Supported by Fundamental Scientific Research Ability Enhancement Project for Young and Middle-Aged Teachers in Universities and Colleges of Guangxi(2019KY0324)
Graduate Project of Guangxi University of Chinese Medicine(xjyb019)
Project of Zhuang Medicine Collaborative Innovation Center(zyx[2015]-01)
Sub-project of Key Laboratory of Pharmacology of Traditional Chinese Medicine(J16072)
College Students’Innovative Entrepreneurial Training Program of Guangxi University of Chinese Medicine(201910600022).
作者简介
Corresponding author:Chenyan LIANG,E-mail:113632962@qq.com;Corresponding author:Yunli TANG,E-mail:214998680@qq.com
