摘要
目的探讨新型高尔基相关蛋白高尔基膜蛋白1(golgi membrane protein 1,GOLM1)在前列腺癌组织中的表达特点及在诊断中的临床价值。方法获取正常前列腺、良性前列腺增生和前列腺癌等不同前列腺组织芯片,采用免疫组化法检测GOLM1蛋白的表达,并分析其表达差异。在人前列腺癌细胞(human prostate cancer cell,PC-3)、DU145和22Rv1三种不同前列腺癌细胞系中,分别采用定量PCR技术和Western blot方法,同时检测GOLM1在mRNA水平和蛋白水平的表达情况。在DU145中,使用免疫荧光技术观察GOLM1在前列腺癌细胞中的定位情况。结果GOLM1在正常前列腺、良性前列腺增生和前列腺癌组织中表达程度不同,其中阳性表达率分别为40.0%(4/10)、50.0%(10/20)和92.0%(46/50),前列腺癌组织中阳性表达率显著高于其他两组,差异有统计学意义(P<0.05)。但病理结果显示,GOLM1的高表达状态与肿瘤Gleason分级和病理分期则差异无统计学意义(P>0.05)。PC-3、DU145和22Rv1三种不同前列腺癌细胞中均见GOLM1表达,激素依赖型前列腺癌细胞22Rv1显著低于激素非依赖型前列腺癌细胞(PC-3和DU145),差异有统计学意义(P<0.05)。GOLM1定位于前列腺癌细胞DU145高尔基体顺面网络结构。结论与正常前列腺和良性前列腺增生相比,GOLM1在前列腺癌中表达明显升高,是一种潜在的肿瘤标志物,进一步研究其在前列腺癌中的作用机制具有重要意义。
Objective To investigate the expression characteristics of a novel Golgi membrane protein1(GOLM1)in prostate cancer tissues and its clinical value in diagnosis.Methods Different prostate tissue specimens,including normal prostate,benign prostatic hyperplasia,and prostate cancer,were collected.The expression of GOLM1 protein was detected via immunohistochemistry and its expression differences were analyzed.Quantitative polymerase chain reaction and western blotting were used to determine the expression of GOLM1 at the mRNA and protein levels,respectively,in three different prostate cancer cell lines,including PC-3,DU145,and 22 Rv1.In DU145 cells,the localization of GOLM1 was observed using immunofluorescence.Results GOLM1 was expressed differently in normal prostate,benign prostatic hyperplasia,and prostate cancer tissue samples.The positive expression rates were 40.0%(4/10),50.0%(10/20),and 92.0%(46/50),respectively.The positive expression rate in cancer tissue was significantly higher than that in the other two groups(P<0.05).However,the pathological results showed that there was no significant correlation between the high expression of GOLM1 and tumor Gleason grade and pathological stage(P>0.05).GOLM1 expression in hormone-dependent 22 Rv1 prostate cancer cells was significantly lower than that in hormone-independent prostate cancer cells PC-3 and DU145(P<0.05).GOLM1 localized to the cis-plane network structure of the Golgi apparatus in DU145 cells.Conclusion Compared to normal prostate and benign prostatic hyperplasia tissue samples,the expression of GOLM1 was significantly increased in prostate cancer,making it a potential tumor marker.Therefore,it is imperative to further study its mechanism of action in prostate cancer.
作者
韦自卫
王忠
李文智
Wei Ziwei;Wang Zhong;Li Wenzhi(Department of Urology,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai,200011,China;Department of Urology,Jinshan Hospital,Fudan University,Shanghai,201508,China)
出处
《泌尿外科杂志（电子版）》
2022年第1期26-30,共5页
Journal of Urology for Clinicians(Electronic Version)
基金
上海市自然科学基金面上项目(20ZR1431400)
关键词
高尔基膜蛋白1
前列腺癌
表达特征
Golgi membrane protein 1
Prostate cancer
Expression characteristics
作者简介
通信作者:李文智,E-mail:wenjhylee@hotmail.com
