摘要
目的:使用低压低氧动物实验舱建立高原雄性大鼠生殖系统损伤模型,同时探讨模拟不同海拔高度的高原环境对雄性大鼠睾丸组织、精液参数、血气分析、氧化应激水平的影响改变。方法:将60只雄性Wistar大鼠随机分为平原组(当地海拔)、高原模型组Ⅰ(海拔4000 m)、高原模型组Ⅱ(海拔6000 m),饲养14 d。取睾丸组织HE染色后光镜下观察不同海拔高度大鼠睾丸组织病理学改变,取附睾制备精子悬液检测不同海拔高度大鼠的精子活力、畸形率,通过血清学检测大鼠血气、氧化应激水平相关指标。结果:成功建立高原雄性大鼠生殖系统损伤模型后,随着海拔高度的升高,pH、PO_(2)降低,PCO_(2)、HCT、K^(+)、Cl^(-)、Hb升高越明显,Na^(+)变化不明显;生精小管管壁褶皱、生精上皮变薄、生精细胞排列紊乱、数量显著减少、上皮内可见空泡样变增加;精子活率、浓度下降,精子畸形率上升;MDA浓度升高,SOD活力降低。结论:模拟海拔高度为4000 m时成功建立高原大鼠生殖系统损伤模型,随设定海拔高度升高至6000 m,模拟海拔6000 m时会对大鼠生殖系统机能产生较为严重的损害影响。
Objective:To establish a model of reproductive system injury in male rats at high altitude using the low-pressure hypoxic animal laboratory and study the changes in the testicular tissue,semen parameters,blood gas and oxidative stress in male rats at different altitudes.Methods:Sixty male Wistar rats were randomly assigned to be raised on the plains(the plains group,n=20),at an altitude of 4000 m(the plateau model groupⅠ,n=20),or at an altitude of 6000 m(the plateau model groupⅡ,n=20)for a spermatogenic cycle of 14 days.After establishment of the model of high-altitude reproductive system injury,the testis tissues of the rats were harvested for HE staining and observed for histopathological changes under the light microscope,and their epididymedes collected for preparation of sperm suspension and detection of sperm motility,sperm count and the percentage of morphologically abnormal sperm(MAS).The blood gas level and oxidative stress-related indexes in different groups were also measured using the serological test.Results:With the elevation of altitude,the levels of pH and PO_(2)were decreased,those of PCO_(2),Hct,K^(+),Cl^(-)and Hb increased markedly,while that of Na^(+)exhibited no significant change.The model rats also showed folded spermatogenic tubule walls,thinned spermatogenic epithelia,disorderly arranged and reduced number of spermatogenic cells,and increased vascuolization in the spermatogenic epithelia,with decreased sperm motility and count,increased percentage of MAS,elevated concentration of malondialdehyde(MDA)and reduced activity of superoxide dismutase(SOD).Conclusion:A model of reproductive system injury was successfully established in male rats at a simulated altitude of 4000 m.With increasing of the altitude to 6000 m,oxidative damage to the testicular tissue was aggravated,sperm motility decreased,and the percentage of MAS increased,indicating that an altitude of 6000 m may cause serious damage to the rat reproductive system.
作者
常德辉
孔飞燕
姜卫
李富东
康印东
周昕
丁豪帅
张斌
CHANG De-hui;KONG Fei-yan;JIANG Wei;LI Fu-dong;KANG Yin-dong;ZHOU Xin;DING Hao-shuai;ZHANG Bin(Department of Urology,the 940th Hospital of PLA Joint Logistics Support Force,Lanzhou,Gansu 730050,China;Department of Urology,Fengtai Hospital of Integrated Traditional Chinese and Western Medicine,Beijing 100072,China;Department of Recuperation 1,Hangzhou Special Service Sanatorial Center of PLA Air Force,Hangzhou,Zhejiang 310007,China;Gansu University of Chinese Medicine,Lanzhou,Gansu 730000,China)
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2020年第12期1068-1073,共6页
National Journal of Andrology
基金
全军高原科研攻关课题(LZ13GY01)
甘肃省自然科学基金(18JR3RA406)
兰州市科技计划项目(2016-2-76)
关键词
高原
模型
氧化应激
生殖损伤
血气分析
大鼠
plateau
model
oxidative stress
reproductive damage
blood gas analysis
rat
作者简介
常德辉(1971-),男,甘肃兰州市人,主任医师,硕士,从事泌尿外科学专业。Email:chdhui@163.com;通讯作者:张斌,Email:1265243155@qq.com
