摘要
目的:研究讨论男性不育患者生殖支原体感染对精子DNA碎片化的影响及其临床应用价值。方法:对生殖支原体感染阳性的100例男性不育患者(患者组)和生殖支原体感染阴性的200例男性不育患者(对照组)同时进行流式细胞仪检测精子DNA碎片化与精液常规参数检查;患者组进行抗菌药物阿奇霉素治疗并检测治疗后的精子DNA碎片化情怳。结果:研究结果表明,患者组的精子DNA碎片化指数(DFI)为(40.1±9.9)%,对照组精子DFI为(5.4±4.9)%,两组差异有统计学意义(P<0.01);患者组的精子DNA碎片化指数(DFI)为(40.1±9.9)%,抗菌药物治疗后的患者组精子DFI为(19.0±9.0)%,两组差异有统计学意义(P<0.01);对比患者治疗前后的精子浓度和活力,差异无统计学意义;而治疗后的不活动精子数明显减少,差异有统计学意义(P<0.05)。结论:生殖支原体感染后的男性不育患者,其精子DNA碎片化程度显著高于未感染生殖支原体男性不育患者,通过抗菌药物治疗可以有效降低感染所造成的精子DNA碎片化水平,精子DNA碎片化分析在生殖支原体感染的男性不育诊疗过程中比精液常规参数分析更有意义。
Objective:To study the impact of mycoplasma genitalium(Mg)infection on sperm DNA fragmentation in male infertility patients and its clinical significance.Methods:We performed flow cytometry and routine semen examination for 100 male infertility patients with Mg infection(the case group)and 200 infertile men without Mg infection(the control group).We treated the patients with the antibacterial drug Azithromycin and examined their sperm DNA fragmentation index(DFI)by flow cytometry after treatment.Results:The baseline sperm DFI was dramatically higher in the case group than in the normal controls[40.1±9.9]%vs[5.4±4.9]%,(P<0.01),but reduced to(19.0±9.0)%after antibacterial medication(P<0.01).The count of immotile sperm was significantly decreased in the infertility patients after treatment(P<0.05),but no statistically significant difference was observed in sperm concentration and motility before and after treatment(P>0.05).Conclusion:The sperm DFI is significantly higher in male infertility patients with Mg infection than in normal men,and antibacterial therapy can effectively reduce the level of Mg infection-induced sperm DNA fragmentation.The analysis of sperm DNA fragmentation plays a more significant role than routine semen examination in the diagnosis and treatment of male infertility with Mg infection.
作者
郑渠
钟影
ZHENG Qu;ZHONG Ying(Department of Laboratory,Chengdu Xinan Hospital of Gynecology,Chengdu,Sichuan 610000,China)
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2022年第11期1020-1025,共6页
National Journal of Andrology
基金
四川省医学青年创新科研课题计划(Q19039)
关键词
男性不育
生殖支原体
精子
DNA碎片化
male infertility
mycoplasma genitalium
sperm
DNA fragmentation
作者简介
郑渠(1980-),女,四川渠县人,副主任技师,本科,从事医学检验专业;通讯作者:郑渠,Email:2911566919@qq.com
