摘要
利用苜蓿雄性不育系杂交制种是提高苜蓿杂交种产量和品质的重要途径。研究团队在草原1号杂花苜蓿群体中鉴定出一株雄性不育株,通过回交转育培育出遗传稳定的苜蓿雄性不育系MS-4。为探究其雄性不育性状形成的分子机制,对雄性可育(MF)和雄性不育(MS)苜蓿花蕾进行了比较转录组学分析。采用RNA-seq技术,鉴定出花粉发育3个阶段的MF和MS之间的差异表达基因(DEGs)共15435个;经GO和KEGG功能富集分析发现,在MF和MS花蕾发育过程中参与昼夜节律(12个)、转录因子(12个)、花粉壁发育(6个)和类黄酮生物合成(12个)的关键基因差异表达显著,为苜蓿雄性不育的分子机制的深入研究提供了新线索。
Hybrid seed production through alfalfa male sterile line is an important way to improve the yield and quality of alfalfa hybrids.The research team identified a male-sterile mutant from the Medicago varia Martin.cv Caoyuan No.1 population,and cultivated a genetically stable alfalfa male-sterile line MS-4 through backcross breeding.In order to explore the molecular mechanism of its male sterility traits,the comparative transcriptomic analysis of male-fertile(MF)and male-sterile(MS)alfalfa flower buds was performed.Using RNA-seq technology,a total of 15435 differentially expressed genes(DEGs)between MF and MS flowers in three stages of pollen development were identified.GO and KEGG functional enrichment analysis revealed the key branch point genes involved in circadian rhythm(12),transcription factors(12),pollen wall development(6),and flavonoid biosynthesis(12)were significantly differentially expressed during the MF And MS flower bud development.The findings provided new clues for the in-depth study on the molecular mechanism of male sterility in alfalfa.
作者
范文强
王佳
高翠萍
高霞
石凤翎
FAN Wenqiang;WANG Jia;GAO Cuiping;GAO Xia;SHI Fengling(College of Grassland Resources and Environment,Inner Mongolia Agricultural University,Hohhot 010011,China;Department of Pharmacy,Baotou Medical College,Baotou 014040,China)
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2022年第5期93-102,共10页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自治区科技重大专项项目(2021ZD0031)
国家自然科学基金项目(32160323)
作者简介
范文强(1992—),男,博士研究生,主要从事牧草遗传育种方面的研究;通信作者:石凤翎,E-mail:sfl0000@126.com
