摘要
目的了解南京市德国小蠊对拟除虫菊酯类杀虫剂的抗性水平,建立一种快捷方便的抗性基因检测分型方法,对kdr(knockdown resistance,kdr)基因突变进行检测和分型。方法利用圆球法检测高效氯氰菊酯、氯菊酯、胺菊酯以及右旋反式氯丙炔菊酯对德国小蠊的半数击倒时间(KT),PCR扩增检测kdr基因突变情况。针对1059位氨基酸位点的碱基突变设计引物建立ARMS-PCR检测方法。结果在100 mg/m剂量时,氰菊酯、氯菊酯、胺菊酯和右旋反式氯丙炔菊酯对德国小蠊试虫的KT值分别20、19、17和8 min。同时在kdr基因的1058-1059氨基酸位点处检测到6种突变基因型,并成功地建立了针对D1059K突变基因型的ARMS-PCR检测方法。结论采集到的德国小蠊对拟除虫菊酯类杀虫剂产生了不同程度的抗性,且其kdr基因存在着多种新型突变位点。
Objective To learn the resistance of Blattella germanica to the pyrethroid insecticides in Nanjing,and to establish a simple,sensitive detection and typing method for the kdr(knockdown resistance)gene mutations.Methods The ball method was used to detect the median knockdown time(KT)of the beta-cypermethrin,permethrin,tetramethrin and dextro-trans-chlorpropinylmethrins,and the mutations of the kdr gene were detected by PCR amplification.The primers were designed for the mutation at the amino acid position 1059 to establish an ARMS-PCR detection method.Results At the dose of 100 mg/m~3,the KTs of beta-cypermethrin,permethrin,tetramethrin and dextro-trans-chlorpropinylmethrins to Blattella germanica were 20,19,17 and 8 minutes,respectively.Six mutation genotypes were detected at the amino acid positions 1058-1059 of the kdr gene,and an ARMS-PCR detection method for the D1059 K mutation genotype was successfully established.Conclusion Blattella germanica collected in the field developed different degrees of resistance to pyrethroid insecticides,and some new mutations in the kdr gene were found.
作者
吕瑞辰
谭伟龙
齐永
朱长强
艾乐乐
韩招久
LYU Rui-chen;TAN Wei-long;QI Yong;ZHU Chang-qiang;AI Le-le;HAN Zhao-jiu(Center for Disease Control and Prevention of Eastern Theater Command,Nanjing,Jiangsu 210002,China)
出处
《中华卫生杀虫药械》
CAS
2022年第1期68-71,共4页
Chinese Journal of Hygienic Insecticides and Equipments
基金
病原生物生物安全重点实验室开放基金(编号:SKLPBS1526)
国家自然科学基金(编号:U1602223)
作者简介
吕瑞辰(1989-),河北任丘市人,男,助理研究员,主要从事病媒生物防治研究工作;通信作者:谭伟龙,E-mail:njcdc@163.com;通信作者:韩招久,E-mail:zhaojiu_han@163.com
