摘要
[目的]研究暗紫贝母超临界CO_(2)挥发油中的化学成分及其含量的测定.[方法]采用超临界CO_(2)萃取法提取暗紫贝母挥发油,通过气相色谱-质谱法(GC-MS)结合NIST谱库检索对其化学成分进行鉴定,同时采用峰面积归一法对各成分的相对百分含量进行测定.[结果]GC-MS分析显示,从暗紫贝母挥发油中共分离得到39个组分,并鉴定其中32种成分,包括酯类化合物(26%)、醇类化合物(21%)、烷烃类化合物(20%),相对百分含量较高的有1-二十三醇(3.61%),七氟丁酸三十四烷基酯(3..59%)和反式-(2,3-二苯基环丙基)甲基苯基亚砜(3.24%)等.[结论]本研究完善了暗紫贝母挥发油的成分及其相关含量,为暗紫贝母的开发利用提供依据.
[Objective]To study the chemical constituents of supercritical CO_(2)volatile oil from Fritillaria unibracteata Hsiao et K.C.Hsia and its determination.[Methods]The volatile oil of Fritillaria ussuriensis was extracted by supercritical CO_(2)extraction,and its chemical components were identified by gas chromatography-mass spectrometry(GC-MS)combined with NIST spectral library retrieval.At the same time,the relative percentage content of each component was determined by peak area normalization method.[Results]GC-MS analysis showed that 39 components were separated from the volatile oil of Fritillaria unibracteata Hsiao et K.C.Hsia,and 32 components were identified,including esters(26%),alcohols(21%),alkanes(20%).The relatively high content of 1-tricosanol(3.61%),tetratriacontyl heptafluorobutyrate(3.59%)and trans-(2,3-diphenylcyclopropyl)methylphenyl sulfoxide(3.24%).[Conclusion]This study improved the components and related contents of the volatile oil from Fritillaria unibracteata Hsiao et K.C.Hsia,and provided a basis for the development and utilization of Fritillaria unibracteata Hsiao et K.C.Hsia.
作者
郭嘉鑫
雷有庭
韩鸿萍
许立秀
陈志
GUO Jia-xin;LEI You-ting;HAN Hong-ping;XU Li-xiu;CHEN Zhi(College of life sciences,Qinghai Normal University,Xining 810016,China;Key Laboratory of Medicinal Animal and Plant Resources of Qinghai-Tibetan Plateau in Qinghai Province,Qinghai Normal University,Xining 810016,China;Forestry and Forest Grass Station of Huzhu Tv Autonomous County Qinghai Province,Haidong 810600,China;College of Chemistry and Chemical Engineering,Qinghai Normal University,Xining 810016,China)
出处
《青海师范大学学报（自然科学版）》
2022年第4期68-76,87,共10页
Journal of Qinghai Normal University(Natural Science Edition)
基金
青海省重点研发与转化计划(2022-SF-142)
青藏高原药用动植物资源重点实验室(2020-ZJ-Y04)
青海省财政改革发展资金新技术推广项目(川贝母种苗繁育与种植技术推广)
关键词
暗紫贝母
挥发油
超临界CO_(2)萃取技术
气相色谱-质谱联用法
Fritillaria unibracteata Hsiao et K.C.Hsia
volatile oil
supercritical CO_(2)extraction technology
gas chromatography-mass spectrometry
作者简介
郭嘉鑫(1998-),女,山西大同人,硕士生.研究方向:植物生物化学;通信作者:陈志(1963-),男,四川乐山人,博士,教授.研究方向:天然产物分离及药理学研究.
