摘要
目的探讨结直肠癌(CRC)患者miR-133b、核孔蛋白153(NUP153)的表达及其临床意义。方法选取2018年8月-2019年8月在河北北方学院附属第一医院收治的153例CRC患者,取手术后切除的癌组织及其对应的癌旁正常组织。采用实时荧光定量PCR(qRT-PCR)法测定CRC组织及癌旁正常组织中miR-133b的表达情况,采用免疫组化法检测CRC组织及癌旁正常组织中NUP153表达水平;分析miR-133b、NUP153与患者临床病理参数的相关性。以CRC组织中miR-133b表达水平平均数将其分为miR-133b高表达组(n=76)和低表达组(n=77),以NUP153蛋白表达情况将其分为NUP153阳性表达组(n=81)和阴性表达组(n=72),对所有CRC患者进行2年随访,Kaplan-Meier方法分析miR-133b、NUP153表达对CRC患者预后的影响;采用COX回归分析影响CRC患者预后不良的危险因素。结果CRC组织中miR-133b的表达水平(0.77±0.12)显著低于癌旁正常组织(1.08±0.56),NUP153阳性表达率(52.94%)显著高于癌旁正常组织(22.22%),差异均有统计学意义(t=6.695,χ^(2)=30.774,P均<0.001)。miR-133b、NUP153表达情况与浸润深度、组织分化程度、区域淋巴结转移有关,差异均有统计学意义(χ^(2)=26.081、34.864、45.713,15.918、39.080、51.425,P均<0.001)。CRC组织中miR-133b表达与NUP153表达有关,差异有统计学意义(χ^(2)=10.006,P=0.002)。Kaplan-Meier结果显示,miR-133b低表达组2年内总生存率、无进展生存率为61.0%、52.0%,低于高表达组(90.8%、69.7%),NUP153阳性表达组2年内总生存率、无进展生存率为66.7%、50.0%,低于阴性表达组(84.0%、70.4%),差异均有统计学意义(P均<0.05)。多因素COX分析表明,miR-133b低表达、NUP153阳性是影响CRC患者预后不良的危险因素(P均<0.05)。结论CRC患者miR-133b低表达,NUP153高表达,二者与患者部分临床病理参数及预后有关,可作为评估患者预后的潜在生物标志物。
Objective To investigate the expression and clinical significance of miR-133b and nucleoporin 153(NUP153)in patients with colorectal cancer(CRC).Methods A total of 153 patients with CRC who were admitted to the First Affiliated Hospital of the Hebei North University from August 2018 to August 2019 were selected,and the cancer tissues resected after surgery and their corresponding adjacent normal tissues to the cancer were taken.The expression of miR-133b in CRC tissues and adjacent normal tissues to cancer was determined by real-time fluorescent quantitative PCR(qRT-PCR);the expression level of NUP153 in CRC tissue and normal tissue adjacent to cancer was detected by immunohistochemical method;the correlation between miR-133b,NUP153 and patients'clinicopathological parameters were analyzed.Based on the average expression level of miR-133b in CRC tissues,they were divided into miR-133b high expression group(n=76)and low expression group(n=77),and according to NUP153 protein expression in CRC tissues,they were divided into NUP153 positive expression group(n=81)and negative expression group(n=72);all CRC patients were followed up for 2 years,the effects of miR-133b and NUP153 expression on the prognosis of patients with CRC were analyzed by Kaplan-Meier method,and the risk factors affecting the poor prognosis of CRC patients were analyzed by COX regression analysis.Results In CRC tissues,the expression level of miR-133b(0.77±0.12)was significantly lower than that in adjacent normal tissues(1.08±0.56),and the positive expression rate of NUP153(52.94%)was significantly higher than that in adjacent normal tissues(22.22%);the differences were statistically significant(t=6.695,χ^(2)=30.774;P all<0.001).The expressions of miR-133b and NUP153 were related to depth of invasion,degree of tissue differentiation and regional lymph node metastasis,the differences were statistically significant(χ^(2)=26.081,34.864,45.713;15.918,39.080,51.425;P all<0.001).The expression of miR-133b in CRC tissue was related to the expression of NUP153(χ^(2)=10.006,P=0.002).Kaplan-Meier results showed that the 2-year overall survival rate and the progression free survival rate of the miR-133b low expression group were 61.0%and 52.0%,respectively,which were lower than that of the high expression group(90.8%,69.7%),and the 2-year overall survival rate and the progression free survival rate of the NUP153 positive expression group were 66.7%and 50.0%,respectively,which were lower than that of the negative expression group(84.0%,70.4%);the differences were statistically significant(P all<0.05).Multivariate COX analysis showed that low expression of miR-133b and positive NUP153 were risk factors affecting the poor prognosis of CRC patients(P all<0.05).Conclusion The low expression of miR-133b and high expression of NUP153 in CRC patients were related to some clinicopathological parameters and prognosis,which could be used as potential biomarkers to evaluate the prognosis of patients.
作者
王晓静
高会斌
高咏梅
杜三军
吴忱思
WANG Xiaojing;GAO Huibin;GAO Yongmei;DU Sanjun;WU Chensi(Department of Gastroenterology,the First Affiliated Hospital of Hebei North University,Zhangjiakou,Hebei 075000,China;Department of Gastroenterology,the Fourth Hospital of Hebei Medical University,Shijiazhuang,Hebei 050000,China)
出处
《热带医学杂志》
CAS
2023年第6期803-808,共6页
Journal of Tropical Medicine
基金
河北省卫生健康委医学科学研究课题计划(20180494)
作者简介
王晓静(1992-),女,硕士研究生,医师,研究方向:胃肠道肿瘤及常见病;通信作者:高会斌,E-mail:gaogaoyun.cool@163.com
