摘要
W1-2菌株是以好氧活性污泥为菌源,以四溴双酚A(tetrabromobisphenol A,TBBPA)驯化筛选得到的一株新型好氧降解菌株.16S rDNA序列表明,W1-2菌株属于假单胞菌属(Pseudomonas sp.),主要以酶降解的模式去除TBBPA.在30℃、pH=7、150 r/min和无其他碳源辅助的条件下,W1-2菌株对10 mg/L TBBPA 5 d的好氧降解率可达91.4%.温度、转速、pH值及TBBPA的质量浓度均会影响W1-2菌株的降解特性,其中pH值对降解率的影响最大.W1-2菌株最适宜降解和生长的环境条件为150 r/min、30~35℃,TBBPA质量浓度为10 mg/L和pH=8.此外,W1-2菌株也是为数不多的无需其他碳源支持、能在高TBBPA质量浓度(30 mg/L)和低氧(0 r/min)条件下仍保持高降解能力的好氧降解菌株.对W1-2菌株的研究,为探究好氧环境下能降解TBBPA的微生物的修复提供了新的视角.
A novel strain,namely W1-2,was acclimatised and isolated from aerobic activated sludge in a mineral salt medium with tetrabromobisphenol A(TBBPA).Strain W1-2 was identified as Pseudomonas sp.according to the 16S rDNA sequence.After 5 days of aerobic incubation,the degradation rate of 10 mg/L TBBPA was 91.4%at 30℃,pH=7,and 150 r/min.The temperature,rotation speed,pH and TBBPA concentration could influence the degradation rate,among which pH was the most influential parameter.The optimal conditions for bacterial degradation and the growth of strain Wl-2 were 150 r/min,30~35℃,initial TBBPA concentration of 10 mg/L,and pH=8 according to a single-factor experiment.Strain W1-2 had an excellent degradation ability at high levels of TBBPA(30 mg/L)and under micro-oxygen conditions(0 r/min)without the support of other carbon sources.This study on the degradation characteristics of strain W1-2 provides a new perspective to explore the microbial removal of TBBPA in aerobic environments.
作者
杨书娴
胡星
YANG Shuxian;HU Xing(School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China)
出处
《上海大学学报（自然科学版）》
CAS
CSCD
北大核心
2022年第1期57-66,共10页
Journal of Shanghai University:Natural Science Edition
关键词
好氧微生物降解
四溴双酚A
假单胞菌属
最优条件
aerobic microbial degradation
tetrabromobisphenol A(TBBPA)
Pseudomonas sp.
optimal parameters
作者简介
通信作者:胡星(1969—),女,副教授,博士,研究方向为环境污染物的微生物治理.E-mail:xhu@shu.edu.cn
