摘要
目的基于JAK2/STAT3信号通路探讨大黄素保护AR42J胰腺腺泡细胞损伤的作用机制。方法将AR42J细胞以1×105/mL铺于6孔板,分为对照组、雨蛙素组(雨蛙素10-8 mol/L)、大黄素组(终浓度0.25、0.5、1.0mg/L),通过碘-淀粉比色法淀粉酶(AMS)试剂盒测定细胞上清淀粉酶活力水平,确定雨蛙素刺激AR42J胰腺腺泡细胞损伤的收样时间;ELISA法检测TNF-α活力;Western blot法分析JAK2/STAT3通路相关蛋白的表达情况。结果①与对照组比较,雨蛙素组中的AMS、TNF-α、JAK2/STAT3通路相关蛋白表达量显著提高,差异有统计学意义(P<0.05)。②与雨蛙素组比较,大黄素各干预组可显著降低雨蛙素诱导的胰腺腺泡细胞损伤的淀粉酶活力、TNF-α含量、JAK2和STAT3的mRNA相对表达量,差异有统计学意义(P<0.01)。③大黄素0.5、1.0 mg/L干预组的作用优于0.25 mg/L干预组,差异有统计学意义(P<0.001)。结论大黄素能够减轻雨蛙素诱导的胰腺腺泡细胞损伤,其作用机制可能与调控JAK2/STAT3信号通路有关。
Objective To investigate the mechanism of emodin protecting AR42 J cells from injury based on JAK2/STAT3 singnaling pathway.Methods AR42 J cells were plated in 6-well plates at a concentration of 1×105 cells/mL,and divided into three groups:control group,cerulein group(cerulein 10-8 mol/L),and emodin groups(final concentration 0.25 mg/L,0.5 mg/L and 1 mg/L).The iodine-starch colorimetric amylase(AMS)kit was used to determine the amylase activity level of the cell supernatant,and the sample collection time of cerulein stimulated AR42 J cell model was determined;the TNF-αactivity was detected by ELISA;and the JAK2/STAT3 pathway related protein expression was analysed by Western blot.Results①Compared with the control group,the levels of AMS,TNF-α,JAK2/STAT3 pathway related protein expression and other inflammatory indicators in the cerulein group significantly increased(P<0.05).②Compared with cerulein group,in the emodin groups amylase activity,TNF-αcontent and relative mRNA expression of JAK2 and STAT3 in pancreatic acinar cell injury induced by cerulein reduced significantly(P<0.01).③Emodin at the concentration of 0.5 mg/L and 1.0 mg/L had better invtervention effects than emodin at the concentration of 0.25 mg/L,and the difference was statistically significant(P<0.001).Conclusion Emodin can reduce the injury of pancreatic acinar cells induced by cerulein,and its mechanism may be related to the regulation of JAK2/STAT3 signaling pathway.
作者
杨小芳
宋雅楠
游丽娇
耿欢
孙芳园
雷鸣
YANG Xiaofang;SONG Yanan;YOU Lijiao;GENG Huan;SUN Fangyuan;LEI Ming(Department of Intensive Care Medicine,The Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China;Central Laboratory,The Seventh People’s Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200137,China)
出处
《上海中医药杂志》
2020年第5期81-86,共6页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(8197141543)
上海市卫健委中医特色诊疗技术提升项目(zyjx-2017021)
上海市浦东新区卫健委临床高原学科建设项目(PWYgy2018-01)
上海市浦东新区卫生系统领先人才培养计划项目(PWR12019-02)
作者简介
杨小芳,女,硕士研究生,主要从事中西医结合治疗危重病研究工作。;通信作者:雷鸣,主任医师,硕士研究生导师。E-mail:leiming6891@163.com
