摘要
目的观察中药首乌藤联合应用黄芪对刀豆蛋白A(ConA)诱导的免疫性肝损伤模型小鼠的影响。方法健康ICR小鼠(SPF级)70只,随机分为正常组(等量0.9%NaCl溶液)、模型组(等量0.9%NaCl溶液)、正常+低剂量首乌藤组(首乌藤药液2 g·kg^(-1)·d^(-1))、正常+高剂量首乌藤组(首乌藤药液6 g·kg^(-1)·d^(-1))、模型+低剂量首乌藤组(首乌藤药液2 g·kg^(-1)·d^(-1))、模型+高剂量首乌藤组(首乌藤药液6 g·kg^(-1)·d^(-1))及模型+高剂量首乌藤+黄芪组(首乌藤药液2 g·kg^(-1)·d^(-1)+黄芪药液6 g·kg^(-1)·d^(-1)),每组10只。各组均每日灌胃1次,连续10 d。末次灌胃后模型组、模型+低剂量首乌藤组、模型+高剂量首乌藤组、模型+高剂量首乌藤+黄芪组予ConA 10 mg·kg-1尾静脉注射诱导免疫性肝损伤模型,8 h后处死各组小鼠并取材。采用HE染色法观察肝脏病理变化,生化法检测小鼠肝功能,包括丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、丙二醛(MDA)、单胺氧化酶(MAO)、谷胱甘肽S-转移酶(GST)、直接胆红素(DBIL)、间接胆红素(IBIL),小鼠血脂包括总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)。结果与正常组比较,模型组小鼠的肝质量、肝脏指数、ALT、AST、MDA和MAO升高(P<0.05),TG和GST降低(P<0.05),肝脏HE染色显示炎性细胞浸润。与模型组比较,模型+低剂量首乌藤组ALT、AST升高(P<0.05),肝脏HE染色可见炎性细胞浸润增加;模型+高剂量首乌藤组ALT、AST、MAO明显升高(P<0.05),MDA升高(P<0.05),GST降低(P<0.05),肝脏HE染色可见炎性细胞浸润加重并伴有部分肝细胞坏死。与模型+高剂量首乌藤组比较,模型+高剂量首乌藤+黄芪组体质量、肝质量、肝脏指数、ALT、AST、MDA、MAO明显降低(P<0.05),GST明显升高(P<0.05),肝脏HE染色未见明显炎性细胞浸润和肝细胞坏死。结论首乌藤会进一步破坏免疫性肝损伤小鼠的肝脏功能,联合黄芪则可产生一定的保护作用。
Objective To observe the effect of Polygoni Multiflori Caulis(PMC)and PMC plus Astragali Radix(AR)on concanavalin A(ConA)induced immunological liver injury(ILI)in model mice.Methods Seventy healthy ICR mice(SPF grade)were randomly divided into normal group,model group,normal group plus high-dose or low-dose PMC,model group plus high-dose or low-dose PMC,model group plus high-dose PMC and AR,with 10 mice in each group.After 10 consecutive days of intragastric administration,ConA with 10 mg·kg^(-1)injected intravenously was used to induce the ILI model at the last intragastric administration in all groups except the normal groups.8 hours later,the mice were sacrificed and samples were collected.The pathological changes of liver were observed by HE staining.Liver function(ALT,AST,MDA,MAO,GST,DBIL,IBIL)and blood lipid(TC,TG,LDL-C)were detected by biochemical method.Results Compared with the normal group,liver weight,liver index,ALT,AST,MDA and MAO of the model group were increased(P<0.05),while TG and GST were decreased(P<0.05).Liver HE staining showed a large number of infiltrated inflammatory cells in the model group.After PMC given(high or low doses),ALT,AST,MDA and MAO levels were increased significantly(P<0.05),while GST was decreased(P<0.05).Additionally,HE staining showed more inflammatory cell infiltration with partial necrosis of liver cells in the model group plus high-dose PMC.However,ILI mice administrated with combination of high-dose PMC and AR,the liver weight,liver index,ALT,AST,MDA and MAO were decreased(P<0.05),but GST was increased(P<0.05).Liver HE staining also showed less inflammatory cell infiltration and necrocytosis.Conclusion PMC can further destroy the liver function of ILI mice,while RA can produce certain protective effect.
作者
陶丽宇
陈晓宏
高月求
TAO Liyu;CHEN Xiaohong;GAO Yueqiu(Geriatrics Department,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Hepatology Department,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《上海中医药杂志》
2022年第8期107-112,共6页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市科委2020年科技创新行动计划医学创新研究专项(20Z21900100)
上海中医药大学附属曙光医院“四明青年基金”项目(SGKJ-201908)
关键词
药物性肝损伤
免疫性肝损伤
首乌藤
黄芪
小鼠模型
中药研究
drug-induced liver injury
immunological liver injury
Polygoni Multiflori Caulis
Astragali Radix
mouse model
traditional Chinese herbal medicine research
作者简介
陶丽宇,女,博士研究生,主治医师,主要从事中医药治疗老年疾病研究工作;通信作者:高月求,主任医师,博士研究生导师,E-mail:gaoyueqiu@hotmail.com
