摘要
目的观察电针"委中"穴后的血清对大鼠腰多裂肌卫星细胞增殖、胶原蛋白I(Collagen I)、基质金属蛋白酶2(MMP2)表达的影响,从血清学角度探讨电针"委中"穴促进多裂肌损伤后修复的部分作用机制。方法25只SPF级雄性SD大鼠,随机分为正常组、模型组、电针组,每组8只。腰4-5节段多裂肌注射0.5%的布比卡因建立多裂肌损伤模型。选取双侧"委中"穴进行电针治疗(20 min/次,1次/日,2/100 HZ,1-2 mA,共3次);正常组、模型组不予电针干预,第4d收集各组大鼠血清,制备无菌针刺血清。使用各组针刺血清培养原代MSCs,CCK-8检测MSCs增殖情况;WB检测Collagen I、MMP2蛋白表达情况。结果与正常血清组比较,模型血清组MSCs增殖速度加快(P<0.01),Collagen I、MMP2含量升高(P<0.01,P<0.05);与模型血清组比较,电针血清组MSCs增殖速度加快(P<0.01),Collagen I含量明显降低(P<0.01),MMP2含量明显升高(P<0.01)。结论电针"委中"后血清可能通过良性调节细胞外基质中Collagen I、MMP2的表达促进MSCs增殖,加快受损多裂肌的再生修复过程。
Objective To observe the intervention effect of electro-acupuncture(EA)serum at"Weizhong(BL40)"on proliferation of multifidus muscle Satellite Cells(MSCs)injury and expression of extracellular matrix(ECM)components Collagen I,Matrix Metalloproteinases 2(MMP2),so as to discuss the possible mechanism of multifidus muscle regeneration.Methods 25 male Sprague Dawley rats were randomly divided into normal group(NG),model group(MG),EA-BL40 group(EG),every group has 8 rats.Rats in MG and EG were treated with intramuscular injection with 0.5%Bupivacaine at bi-multifidus muscle of lumbar 4 and 5(4 points,100μL for every point).Rats in EG was treated with EA at BL40 for 3 day.No EA intervention was given to the rats in NG and MG.Blood samples of the abdominal artery of rats in the 3 groups were separately collected for extracting serum,the serum were inactivated and filtrated,and then were respectively applied to the Dulbeccos Modified Eagle Media(DMEM)culturing each multifidus muscle MSCs of normal serum,model serum,EA serum for analyzing the EA serum on the proliferation state of MSCs by Cell Counting Kit-8(CCK-8)and the expression of Collagen I,MMP2.Results Compared with the normal serum group,the proliferation level and the expression of Collagen I and MMP2 of MSCs in the model serum group were increased(P<0.01,P<0.01,P<0.05),while in comparison with the model serum group,the proliferation level and the expression of MMP2 of MSCs were significantly increased(P<0.01,P<0.01)and the expression of Collagen I was decreased(P<0.01)in the EA serum group.Conclusion EA-BL 40 can accelerate the proliferation of MSCs and improve the repair of injured multifidus muscle through the ECM components Collagen I and MMP2.
作者
陈玉佩
李晓红
许玥
徐菁
霍则军
张莉
Chen Yupei;Li Xiaohong;Xu Yue;Xu Jing;Huo Zejun;Zhang Li(School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Acupuncture-Moxibustion and Massage Department of Nanyuan Hospital,Beijing 100076,China;School of Life Sciences,Beijing University of Chinese Medicine,Beijing 100029,China;Department of Chinese Medicine,Peking University 3rd Hospital,Beijing,100191,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第4期1324-1329,共6页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然基金委员会面上基金项目(81574052):电针“委中穴”对多裂肌卫星细胞增殖过程中的自噬调控,主持人:霍则军
关键词
细胞外基质
委中
多裂肌
肌卫星细胞
Extracellular matrix
Weizhong
multifidus muscle
muscle Satellite Cells
作者简介
通讯作者:张莉,教授,主要研究方向:针灸临床作用机理研究。
