摘要
目的探讨垂黄清脉冲剂对冠心病稳定型心绞痛(热毒痰瘀证)患者NF-κB信号通路的调控。方法本试验研究方法采用单盲、随机、对照法,选取就诊于宁夏医科大学总医院临床诊断为冠心病稳定型心绞痛(热毒痰瘀证)患者94例,将其随机分入对照组48例和试验组46例。两组均给予内科常规西药治疗,对照组加服银丹心脑通软胶囊,试验组加服垂黄清脉冲剂,疗程为3个月。于治疗前、后记录患者的基本情况,并采集血清样本,用酶联免疫吸附测定法(Elisa法)检测血清中TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1、NO浓度水平进行对比,用SPSS22.0将数据进行统计学分析。结果与治疗前比较,两组治疗后Gensini积分、血清中TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1浓度水平均较治疗前显著降低,NO浓度水平显著升高(P<0.05,P<0.01),其中试验组显著程度尤为明显(P<0.01)。与治疗后对照组比较,试验组Gensini积分、血清中TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1浓度水平降低,NO浓度水平升高趋势更明显(P<0.01)。结论垂黄清脉冲剂治疗冠心病稳定型心绞痛(热毒痰瘀证)在改善患者症状;改善冠状动脉管腔狭窄程度;通过降低炎症标志物TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1的水平,减小ET-1/NO比值,从而对NF-κB信号通路进行部分调控,以有效抑制或截断血管内皮的炎症级联反应过程,清除炎症损伤因素以及保护血管内皮功能等方面均有显著优势,临床疗效确切值得肯定和推广应用。
Objective To investigate the regulation of Chui Huang Qing Mai granules on NF-κB signaling pathway in patients with stable angina pectoris(Re Du Tan Yu syndrome).Methods In this study,94 patients with clinically diagnosed coronary heart disease with stable angina(Re Du Tan Yu syndrome)participated in a single-blind,randomized,controlled trial.These patients were from the department and outpatient department of TCM and Cardiology,General Hospital of Ningxia Medical University.All patients were randomly assigned to 48 patients in the control group and 46 patients in the experimental group.This two groups were treated with conventional western medicine,and the control group was treated with Yindan Xinnaotong soft capsule on the basis of conventional western medicine treatment,the experimental group took Chui Huang Qing Mai granules on the basis of conventional western medicine treatment.All patients have a 3-month course of treatment.The researchers recorded the basic conditions of these patients before and after treatment,and collected serum samples from these patients,and then measured the Gensini scores and the serum levels of TNF-α、NF-κB、IL-6、ICAM-1、VCAM-1、ET-1、NO by enzyme-linked immunosorbent assay(Elisa)and compared them.Statistical analysis of data with SPSS22.0.Results Compared with before treatment,the Gensini scores and the serum levels of TNF-α,NF-κB,IL-6,ICAM-1,VCAM-1 and ET-1 were significantly lower in two groups than before treatment.The concentration of NO increased significantly(P<0.05,P<0.01),especially in the experimental group(P<0.01).Compared with the control group after treatment,the levels of TNF-α,NF-κB,IL-6,ICAM-1,VCAM-1 and ET-1 in the serum of the experimental group decreased,and the concentration of NO increased more significantly(P<0.01).Conclusion Chui Huang Qing Mai granules in the treatment of coronary heart disease with stable angina pectoris(Re Du Tan Yu syndrome)in improving patients stenosis;it mainly aims to regulate part of the NF-κB signaling pathway by reducing the levels of inflammatory markers TNF-α,NF-κB,IL-6,ICAM-1,VCAM-1,and decreasing the ratio of ET-1/NO to effectively inhibit or cut off the vascular endothelial inflammatory cascade process,clear inflammatory damage factors and protect vascular endothelial function have significant advantages,the clinical efficacy is definitely worthy of recognition and popularization.
作者
赵凯
叶晓辉
Zhaokai;Yexiaohui(General Hospital of Ningxia Medical University,Yinchuan 750004,China;International Medical Center Hospital of Xi'an,Xi'an 710010,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2020年第5期1437-1443,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
宁夏回族自治区科学技术厅一般研发计划项目(2018BEG03037):中药新配方垂黄冲剂治疗冠心病患者临床应用研究
负责人:赵凯
关键词
垂黄清脉冲剂
稳定型心绞痛
热毒痰瘀证
Chui Huang Qing Mai granules
Stable Angina
Re Du Tan Yu Syndrome
作者简介
通讯作者:叶晓辉,住院医师,主要研究方向:心脑血管及周围血管疾病、风湿、内分泌方向。
