摘要
目的基于数据非依赖性采集的定量蛋白质组学技术(data independent acquisition,DIA)对稳定性冠心病血瘀证患者的血浆蛋白质进行研究分析,初步探索冠心病血瘀证的关键生物学过程与核心靶点。方法选择中国中医科学院广安门医院心血管科收治的5例稳定性冠心病患者作为疾病观察组,同时选取健康成人5人作为对照组,采用DIA蛋白质组学技术对受试者的血浆进行检测,确定蛋白质表达量后进行差异蛋白质表达分析、GO富集分析、KEGG信号通路图查询和蛋白质互作网络分析。结果在差异倍数为1.5倍时,疾病观察组相比于健康对照组,共鉴定到22个蛋白质下调,9个蛋白质上调。进一步的生物信息学分析表明,冠心病血瘀证的生物学过程与补体参与的慢性炎症反应有关,关键信号通路是补体与凝血级联反应的信号通路,核心蛋白质为C反应蛋白、转甲状腺素蛋白、补体因子H、维生素D结合蛋白等。结论冠心病血瘀证的生物学过程与补体参与的慢性炎症反应有关。
Objective Based on the quantitative proteomics of data independent acquisition(DIA)to analyze the plasma proteins of patients with blood stasis syndrome of coronary artery disease(CAD),and explore the key biological process and potential biomarkers.Method Five patients with stable CAD from the department of cardiology,Guang’anmen Hospital,were selected as the observation group,and five healthy adults were selected as the control group.The plasma of the subjects was detected by DIA proteomics technology.After determining the differential protein,we perform differential protein expression analysis,GO enrichment analysis,KEGG signal pathway map query and protein-protein interaction analysis.Results When the fold change was 1.5,compared with the control group,22 proteins in the observation group were down-regulated and 9 proteins were up-regulated.Further bioinformatics analysis showed that the biological process of blood stasis syndrome of SCAD is related to the chronic inflammatory response in which the complement system is involved.The core signal pathway is the complement and coagulation cascade.The core targets are von Willebrand factor,complement factor H.Conclusion This study initially screened the candidate biomarkers for SCAD with blood stasis syndrome as complement factor H.The biological process of the blood stasis syndrome is related to the chronic inflammatory response in which the complement system is involved.
作者
杨光
何浩强
谌子诺
周思远
王阶
Yang Guang;He Haoqiang;Shen Zinuo;Zhou Siyuan;Wang Jie(Guang’anmen Hospital,China Academy of Chinese Medical Science,Beijing 100053,China;College of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2022年第8期3057-3066,共10页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上项目(81974556):冠心病稳定型心绞痛痰瘀互结证多组学网络块整合研究
负责人:王阶
关键词
冠心病
血瘀证
蛋白质组学
DIA
Coronary artery disease
Blood stasis syndrome
Proteomics
DIA
作者简介
通讯作者:王阶,本刊编委,主任医师,主要研究方向:中西医结合防治心血管病。
