摘要
目的基于血清代谢组学研究分析冠心病稳定型心绞痛脾虚痰浊证的差异代谢物和相关代谢通路。方法招募60例冠心病稳定型心绞痛脾虚痰浊证患者和60例同期健康志愿者,采用超高效液相色谱-质谱联用测定两组受试者的血清代谢组学,通过原始谱图和原始数据进行多元统计分析鉴定差异代谢物,进行KEGG代谢通路富集分析。结果冠心病稳定型心绞痛脾虚痰浊组总计60例患者参与研究,对照组总计60例健康志愿者参与研究,两组受试者的一般资料、生化指标均无统计学差异(P>0.05);共鉴定得到18个差异代谢物,其中苯乙醛、正磷酸盐、鸟嘌呤核苷、磷酸二乙酯、2-脱氧-D-葡萄糖酸盐、鸟嘌呤、5-(2-羟乙基)-4-甲基噻唑表达下调,牛黄胆酸、2-丙基戊二酸、8-氨基-7-氧代壬酸、L-酪氨酸、S-磺基-L-半胱氨酸、环己甲酸、紫质胆素原、(R)-3-羟基-2-丁酮、辛酰基葡糖苷酸、褪黑素、A-茄碱表达上调;涉及苯丙氨酸代谢、硫胺素代谢以及嘌呤代谢等代谢通路。结论差异代谢物在一定程度上从微观层面揭示了冠心病稳定型心绞痛脾虚痰浊证的代谢本质以及为冠心病稳定型心绞痛脾虚痰浊证患者临床早期预警提供线索。
Objective To analyse the differential metabolites and related metabolic pathways in stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome by serum metabolomics.Methods This study observed 60 patients with stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome and 60 healthy volunteers in the same period.Liquid chromatography-mass spectrometry(LC-MS)was performed on the serum metabonomics.The differential metabolites were identified by multivariate statistical analysis of the original spectrogram and original data,and enrichment analysis of KEGG metabolic pathway was analyzed.Results A total of 60 patients in the group of stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome participated in the study,and a total of 60 healthy volunteers in the control group participated in the study.There was no statistical difference in general information and biochemical indicators between the two groups(P>0.05);Eighteen differential metabolites were found respectively,including phenylacetaldehyde,orthophosphate,guanosine,diethyl phosphate,2-dehydro-d-gluconate,guanine and 5-(2-hydroxyethyl)-4-methylthiazole down-regulated expression,taurocholate,2-propylglutaric acid,8-amino-7-oxononanoate,l-tyrosine,s-sulfo-l-cysteine,cyclohexanecarboxylic acid,porphobilinogen,(r)-acetoin,octanoylglucuronide,melatonin and solanine up-regulated expression,involving phenylalanine metabolism,thiamine metabolism,purine metabolism.Conclusion The differential metabolites reveal the metabolic essence of stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndrome from the micro level,and can provide clues for clinical early warning of patients with stable angina pectoris of coronary artery heart disease with spleen deficiency and phlegm turbidity syndromet.
作者
袁常斌
姚禹竹
刘勇明
程美佳
谢思梦
鞠业涛
张诗宇
曾乙凡
张会永
杨关林
闵冬雨
Yuan Changbin;Yao Yuzhu;Liu Yongming;Cheng Meijia;Xie Simeng;Ju Yetao;Zhang Shiyu;Zeng Yifan;Zhang Huiyong;Yang Guanlin;Min Dongyu(The First Clinical College of Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;Anshan Hospital of Traditional Chinese Medicine,Anshan 114000,China;The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第9期3105-3113,共9页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委员会面上项目(81974548):健脾祛痰化瘀法调控肠道菌群驱动巨噬细胞糖代谢重编程防治AS机制研究,负责人:杨关林
沈阳市科技计划项目(20-205-4-008):基于肠道菌群多样化探讨“痰瘀论治”稳定性心绞痛的发病机理,负责人:杨关林
国家自然科学基金委员会面上项目(82174114):千金开心散调控剪切力感受器Integrin/YAP/LRP1促进Aβ跨血脑屏障转运防治AD机制研究,负责人:闵冬雨。
关键词
冠心病稳定型心绞痛
脾虚痰浊证
代谢组学
液相色谱-质谱分析
Stable angina pectoris of coronary artery heart disease
Spleen deficiency and phlegm turbidity syndrome
Metabonomics
Liquid chromatography-mass spectrometry
作者简介
通讯作者:杨关林,主任医师,主要研究方向:中医药防治心血管疾病研究;通讯作者:闵冬雨,主任药师,主要研究方向:中医药防治心脑血管疾病机制研究。
