摘要
采用Golden Gate Assembly的方法,将糖化酶基因glaA导入到实验室前期筛选的一株耐高温酿酒酵母S.C HR的基因组中,筛选到了一株同时具有耐高温及产糖化酶特性的功能型酵母COTYT-H。发酵实验结果表明:所构建的COTYT-H菌株能够分泌糖化酶,且能够在高温条件下具有良好的发酵性能,应用该菌株可在减少冷却水用量、糖化酶用量、降低能耗、节约生产成本的同时,提高原料利用率,提升生产效益。
The Golden Gate Assembly method was used to introduce the glucoamylase gene glaA into the genome of a thermotolerant yeasts SC HR which was screened earlier in the laboratory. A functional yeast COTYT with both high temperature resistance and saccharifying enzyme properties was selected. The fermentation experiment results showed that the COTYT-H strain constructed in this research could secrete saccharifying enzymes and had good fermentation performance under high temperature conditions. The application of this strain could reduce the amount of cooling water, the amount of saccharifying enzymes, reduce energy consumption and costs, as well as increase the utilization rate of raw materials and improve production efficiency.
作者
刘辉
何太波
赵国淼
张永昌
张俊奇
王小艳
李凡
佟毅
LIU Hui;HE Tai-bo;ZHAO Guo-miao;ZHANG Yong-chang;ZHANG Jun-qi;WANG Xiao-yan;LI Fan;TONG Yi(COFCO Bio-energy(Zhaodong)Co.,Ltd.,Zhaodong Heilongjiang 151100,China;COFCO Nutrition and Health Institute,Beijing 102209,China;Jilin COFCO Biochemical Co.,Ltd.,Changchun Jilin 130033,China)
出处
《当代化工》
CAS
2020年第8期1703-1706,共4页
Contemporary Chemical Industry
关键词
糖化酶
酵母
乙醇
Glucoamylase1
Yeast
Alcohol
作者简介
刘辉(1972-),女,黑龙江肇东人,高级工程师,硕士,研究方向:谷物发酵生产酒精。E-mail:liuhui06081997@163.com。;通讯作者:佟毅(1963-),男,教授级高级工程师,博士,研究方向:玉米深加工。
