摘要
目的了解一种过氧化氢低温表面消毒液消毒性能,为低温物体表面消毒提供科学依据。方法在-20.1℃~-22.6℃条件下,进行细菌载体定量杀灭试验、病毒灭活试验和低温冻库模拟现场消毒试验,同时测定消毒剂的理化特性和LD_(50)毒性。结果该消毒液pH为5.25,主要杀菌成分为1.91%过氧化氢,性能稳定,对不锈钢、铜和铝有轻度腐蚀,对碳钢有中度腐蚀;在-20.1℃~-22.6℃条件下,5 d未结冰,作用5 min对大肠杆菌的杀灭对数值大于3.00,作用10 min对金黄色葡萄球菌的杀灭对数值大于3.00,作用20 min对脊髓灰质炎病毒Ⅰ型(PV-Ⅰ)疫苗株的灭活对数值大于3.00,经30次模拟现场试验,该消毒液原液作用20 min对金黄色葡萄球菌的杀灭对数值均大于3.00。急性经口毒性LD_(50)大于5000 mg/(kg·bw),属实际无毒级,对家兔的多次皮肤刺激试验属无刺激性,小鼠骨髓嗜多染红细胞微核试验结果为阴性。结论该消毒液能达到杀灭大肠杆菌和金黄色葡萄球菌的要求,能有效灭活脊髓灰质炎病毒Ⅰ型(PV-Ⅰ)疫苗株,无毒对皮肤无刺激,可广泛用于低温物体表面的消毒,有效切断新冠病毒污染的冷链食品接触传播途径。
Objective To understand the disinfection performance of a hydrogen peroxide low-temperature surface disinfectant,and to provide a scientific basis for surface disinfection of low-temperature objects.Methods At the temperature of-20.1℃-22.6℃,the quantitative killing test of bacterial carriers,virus inactivation test and simulated field disinfection test in low-temperature freezer were performed,and the physicochemical features and acute oral toxicity(LD_(50))were determined simultaneously.Results The disinfectant had stable performance when its pH was 5.25 and the main bactericidal component was 1.91%hydrogen peroxide.It had mild corrosion to stainless steel,copper and aluminum,and moderate corrosion to carbon steel.Under the condition of-20.1℃-22.6℃,the disinfectant did not freeze for 5 days,and the killing logarithmic values of Escherichia coli for a contact time of 5 minutes,Staphylococcus aureus for a contact time of 10 minutes,and poliovirus type I(PV-I)vaccine strain for a contact time of 20 minutes were all greater than 3.00.After 30 simulated field tests,the stock solution of this disinfectant killed Staphylococcus aureus for a contact time of 20 minutes,and the killing logarithmic values were all greater than 3.00.The LD_(50)was more than 5,000 mg/(kg.bw),which belonged to the actual non-toxic level.It had no irritation to the skin of white rabbits for many times,and the micronucleus test of polychromatic erythrocytes in mouse bone marrow was negative.Conclusion The properties of the disinfectant can meet the disinfection requirements of killing Escherichia coli and Staphylococcus aureus.The disinfectant can effectively inactivate poliovirus type I(PV-I)vaccine strain,and is non-toxic and non-irritating to the skin.It can be widely used for surface disinfection of low-temperature objects and effectively cut off the route of contact and transmission of cold-chain food contaminated with SARS-CoV-2.
作者
尹进
张莹莹
夏昕
陈贵秋
宋江南
戴俊斌
高琼
阳志文
YIN Jin;ZHANG Yingying;XIA Xin;CHEN Guiqiu;SONG Jiangnan;DAI Junbin;GAO Qiong;YANG Zhiwen(Hunan Provincial Center for Disease Control and Prevention,Changsha,Hunan 410005,China)
出处
《实用预防医学》
CAS
2023年第10期1167-1171,共5页
Practical Preventive Medicine
基金
湖南省卫生健康委科研课题(202212065126,202212064094)
关键词
复方过氧化氢
细菌
病毒
低温
消毒效果
毒性
compound hydrogen peroxide
bacterium
virus
low temperature
disinfection effect
toxicity
作者简介
同为第一作者:尹进(1972-),男,副主任药师,研究方向:消毒与感染控制研究;同为第一作者:张莹莹;通信作者:夏昕,E-mail:19332128@qq.com。
