摘要
目的探讨miR-155对颅脑损伤后小鼠肠黏膜组织中紧密连接蛋白claudin-1表达的影响,分析miR-155对TBI后肠黏膜屏障功能障碍的诊断价值及治疗方向。方法30只C57BL/6小鼠,建立TBI模型后,分为miR-155组和对照组,miR-155组给予AAV-mmu-mir-155-5p inhibitor,对照组给予同等容量生理盐水。干预24 h后处死小鼠取血清及小肠组织进行相关检测。结果miR-155处理组血清中二胺氧化酶(DAO)水平较对照组低,且有统计学差异(P<0.001);光镜下对小肠组织进行病理评分,与miR-155相比,对照组Chiu’s评分明显降低,且有统计学差异(P<0.001);免疫组织化学结果显示miR-155组紧密连接蛋白claudin-1表达明显升高,均匀分布在肠上皮黏膜组织中,对照组claudin-1表达较miR-155组明显减弱,且呈点片状分布,两组间的表达差异有统计学意义(P<0.001)。而两组小鼠肠黏膜组织中occludin的表达镜下观察无明显差异(P>0.05)。结论MiR-155在肠黏膜屏障功能障碍的发生发展中起重要作用,且可能是通过靶向调控claudin-1来影响肠黏膜屏障功能的。
Objective To investigate the effect of miR-155 on the expression of tight junction protein Claudin-1 in intestinal mucosa of mice after brain injury(TBI),and to analyze the diagnostic value and therapeutic direction of miR-155 on intestinal mucosal barrier dysfunction after TBI.Methods Thirty C57BL/6J mice were divided in-to miR-155 group and control group.The miR-155 group was given AAV-mmu-mir-155-5p inhibitor,and the control group was given the same volume of normal saline.After 24 hours of intervention,the mice were euthanized and serum and small intestine tissue were collected for relevant testing.Results The serum Dao level of miR-155 treatment group was lower than that of the control group,and miR-155 could reduce the degree of intestinal injury,and the difference was statistically significant(P<0.001);Pathological scores of small intestine tissues were performed under light microscope,compared with miR-155,the Chiu,s score of the control group was significantly lower(P<0.001);Immunohistochemical results showed that the expression of Claudin-1 in miR-155 group was significantly increased and evenly distributed in the intestinal epithelial mucosa.The expression of Claudin-1 in the control group was significantly lower than that in miR-155 group,and showed a patchy distribution,the expression difference between the two groups was statistically significant(P<0.001).There was no significant difference in the expression of occludin in intestinal mucosal tissue between the two groups(P>0.05).Conclusions miR-155 plays an important role in the development of intestinal mucosal barrier dysfunction,and it may affect the intestinal mucosal barrier function by targeting claudin-1.
作者
雷泓
顾莹
敖德莉
初美玲
宋云林
LEI Hong;GU Ying;AO Deli;CHU Meiling;SONG Yunlin(Department of anesthesiology,the central hospital of Karamay,Karamay,Xinjiang,834000,China;Critical medicine center,the first affiliated hospital of Xinjiang medical university,Urumqi,830054,China)
出处
《新疆医学》
2024年第3期263-267,共5页
Xinjiang Medical Journal
基金
国家自然科学基金(项目编号:81560310)
作者简介
雷泓,女,硕士,副主任医师,研究方向:肠屏障功能障碍及临床麻醉;通信作者:宋云林,男,硕士,主任医师,研究方向:肠屏障功能障碍。E-mail:67869538@163.com。
