摘要
[目的]探讨高丽红参对庆大霉素引起的耳毒性的保护作用及其机制.[方法]取8周龄SD雄性大鼠分为对照组和实验组,对照组大鼠左耳鼓膜内注射给予庆大霉素(剂量20μL,质量浓度为0.5 mg/L,每2 d给药1次,共7次),实验组大鼠在注射给予庆大霉素7 d前开始灌胃给予高丽红参粉溶液(剂量为2 mL,质量浓度为500 mg/kg,每日给药),7 d后开始注射给予庆大霉素,方法与剂量与对照组相同.注射给予庆大霉素前和实验第1,8,15,22,30 d时分别行耳前庭功能和听力功能检查.第30 d检查结束后取大鼠耳蜗前庭器行固定,用扫描电子显微镜和激光共聚焦显微镜观察耳蜗毛细胞、球囊斑和椭圆囊斑毛细胞的形态学变化并进行分析.[结果]实验组实验各时间点时的听力下降幅度均低于对照组,但两组间差异均无统计学意义(P>0.05).前庭功能检查及头偏实验结果显示,对照组注射给予庆大霉素1 d后4只大鼠出现严重头偏(>40℃),3只出现中度头偏(10℃~40℃),而实验组均未出现(P<0.01);提尾实验结果显示,对照组注射给予庆大霉素后4只大鼠出现不能控制的头旋转,而实验组大鼠均未出现(P<0.05);游泳实验结果显示,对照组5只及实验组1只大鼠出现游泳时间延长,两组间差异无统计学意义(P>0.05).扫描电子显微镜和激光共聚焦显微镜检查结果显示,对照组与实验组耳蜗毛细胞和球囊斑/椭圆囊斑毛细胞计数间差异均有有统计学意义(P<0.01).[结论]高丽红参对庆大霉素引起的耳前庭功能损伤具有保护作用,其机制认为可能与保护毛细胞免受损伤和促进毛细胞增殖有关系.
OBJECTIVE To investigate the protective effects of Korean red ginseng(KRG)on ototoxicity induced by gentamicin(GM)and its mechanism.METHODS Male SD rats aged 8 weeks were divided into control group and experimental group.The rats in the control group were injected with GM in the left eardrum(dosage:20μL,mass concentration:0.5 mg/L,once for every 2 days,a total of 7 times).The rats in the experimental group were given the solution of KGR powder by gavage before 7 days of GM injection(dosage:2 mL,mass concentration:500 mg/kg,administration daily),GM was injected to the experimental group after 7 days with the same method and dose as the control group.Vestibular and hearing function were examined before GM injection and on day 1,8,15,22 and 30 of the experiment.The rat cochlea vestibules were taken and fixed after the 30 days examination.Scanning electron microscope and laser confocal microscope were used to observe and analyze the morphological changes of cochlear hair cells,macula sacculi and utricular hair cells.RESULTS The hearing loss in the experimental group was lower than that in the control group at each time point of the experiment,but there was no significant difference between the two groups(P>0.05).The results of vestibular function test and head deviation experiment showed that 4 rats had severe head deviation(>40℃),3 rats had moderate head deviation(10℃-40℃)in the control group 1 day after GM injection,but none occurred in the experimental group(P<0.01).The results of tail lift experiment showed that 4 rats in the control group showed uncontrollable head rotation after GM injection,while the rats in the experimental group did not appear this symptom(P<0.05).Swimming experiment results showed that 5 rats in the control group and 1 in the experimental group showed prolonged swimming time,but there was no significant difference between the two groups(P>0.05).The results of scanning electron microscopy and laser confocal microscopy showed that there were statistically significant differences in the counts of cochlear hair cells and macula sacculi/utricular hair cells between the control and the experimental group(P<0.01).CONCLUSION KRG had protective effects on vestibular dysfunction caused by GM,and its mechanism might be related to protecting hair cells from damage and promoting the proliferation of hair cells.
作者
田春杰
于泳
王莹
李柱虎
TIAN Chunjie;YU Yong;WANG Ying;LI Zhuhu(Department of Pathology and Forensic,Yanbian University College of Medicine,Yanji 133002,Jilin,China)
出处
《延边大学医学学报》
CAS
2019年第4期252-256,共5页
Journal of Medical Science Yanbian University
作者简介
田春杰(1980-),男,硕士研究生,研究方向为分子病理学;通信作者:李柱虎,Email:lizh@ybu.edu.cn.
