摘要
目的:采用高效液相色谱法(HPLC),创建广西产拳卷地钱药材HPLC指纹图谱,与不同产地,品种地钱药材的指纹图谱进行对比分析,通过相似性分析、聚类分析、主成分分析,对其质量进行鉴别、分类和评价。方法:乙腈-0.1%磷酸为流动相,流速1 mL/min,波长270 nm,柱温30℃为条件,建立广西产拳卷地钱及其它共30个地钱类药材指纹图谱。结果:30批广西产地钱类药材供试品的相似度>0.8。聚类分析和主成分分析结果均将药材分为四大类。广西产地钱类药材中的A2、A4、A6、A7、A8、A10、A11、B2、B3、B6、B7、B8、B9、B10、B11、C1、C3、C5、C6、C8产地相比于其他三类产地的相似性更高。主成分分析提取了4个主要成分,特征值λ1的贡献率为58.993%,λ2的贡献率为21.957%,λ3的贡献率为8.654%,λ4的贡献率为5.010%,前4个特征值的累积贡献率达94.606%,其中第一主成分具有最大的特征值。结论:建立了广西产拳卷地钱及其它地钱类药材指纹图谱,并进行了几种地钱指纹图谱的对比,对广西产地钱药材生药学的系统研究进行了补充,为其质量标准的制定提供了实验依据,为广西产拳卷地钱的开发和利用奠定基础。
Objective:This paper adopts HPLC fingerprint method to research Marchantia convoluta Gao et Chang from Guangxi and compared with the fingerprints of different origins and different varieties of medicinal materials.which was identified,classified and evaluated by similarity analysis,hierarchical cluster analysis(HCA),principal component analysis(PCA).Methods:The separation was developed on InertSustain-C18(4.6 mm*250 mm,5μm)by gradient elution with acetonitrile-water(bothcontaining 0.1%phosphoric acid)as mobile phase at a flow rate of 1.0 mL·min-1,the column temperature at 30℃and detection wavelength at 270 nm,and the reference fingerprints were established.Results:The similarity of 30 samples of medicinal herbs from Guangxi was more than 0.8,and the composition is relatively stable.The results of cluster analysis and principal component analysis divide the medicinal materials into four categories,and the analysis results are consistent.Cluster analysis showed that A2,A4,A6,A7,A8,A10,A11,B2,B3,B6,B7,B8,B9,B10,B11,C1,C3,C5,C6 and C8 from Guangxi origin were more similar than those from other three origins.Principal component analysis extracts four main components,the.contribution rate of eigenvalue lambda 1 is 58.993%,the contribution rate of lambda 2 is 21.957%,the contribution rate of lambda 3 is 8.654%,the contribution rate of lambda 4 is 5.010%,and the cumulative contribution rate of the first four eigenvalues is 94.606%.The score has the largest eigenvalue.Conclusion:The fingerprints and comparisons of Marchantia convoluta Gao et produced in Guangxi and other land-based medicines were established,and the relationship between the spectrum and effect was expounded.It supplemented the systematic research on the pharmacology of medicinal materials in Guangxi,To lay the foundation for the development and utilization of Marchantia convoluta Gao et produced by Guangxi.
作者
刘婧曦
植春婷
张淼
谢凤凤
朱华
Liu JingXi;Zhi Chunting;Zhang Miao;Xie Fengfeng;Zhu Hua(Guangxi University of Chinese Medicine,Nanning 530200;Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine,Nanning 530200,China;Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine,Nanning 530200,China;Guangxi Zhuang Autonomous Region Ethnic Medicine Resources and Application Engineering Research Center,Nanning 530200,China)
出处
《壮瑶药研究》
2023年第2期92-98,384-385,共9页
Research of Zhuang and Yao Ethnic Medicine
基金
广西重点研发计划项目(桂科AB21196016)
广西科技基地和人才专项(桂科AD21238031)
广西中医药重点学科壮药学(GZXK-Z-20-64)
关键词
拳卷地钱
地钱
HPLC指纹图谱
Marchantia convoluta Gao et
Marchantia polymorpha L.
fingerprint of HPLC
作者简介
第一作者:刘婧曦,学士,从事中药品种、质量及资源开发;通讯作者:朱华,博士,教授,博士生导师,从事中药品种、质量及资源开发
