摘要
目的探讨茶多酚(TP)对衰老合并雌激素缺乏大鼠记忆能力、海马突触结构及相关蛋白表达的影响。方法60只SD雌性大鼠随机分为假手术(Control,CN)、衰老合并雌激素缺乏(Model,M)、茶多酚低、中、高TP-L、TP-M、TP-H(75,150,300 mg/kg)及维生素E(VE,60 mg/kg),共6组,每组10只。CN组大鼠切除卵巢附近等体积脂肪组织,腹腔注射等量生理盐水,其余各组大鼠行双侧卵巢切除术并持续12 w腹腔注射D-半乳糖[100 mg/(kg·d)]。各干预组大鼠每日灌胃相应受试物,CN和M组大鼠每日灌胃等量蒸馏水,1/d,共12 w。Y迷宫检测大鼠空间记忆能力,透射电镜观察大鼠海马组织突触超微结构并量化分析突触界面曲率、突触间隙、PSD厚度及数密度,Western Blot测定海马组织SYN及PSD-95蛋白表达。结果与CN组比,M组大鼠新异臂进入次数占比和新异臂总路程减少,突触界面曲率降低,突触间隙增大,PSD厚度及数密度降低,SYN和PSD-95蛋白表达下调。与M组相比,TP-H和VE组新异臂次数占比增加,各干预组新异臂总路程均增加;TP-H组突触界面曲率增加,各干预组突触间隙宽度减小,PSD厚度增加,TP-M和TP-H组数密度增加。TP-M、TP-H及VE组大鼠海马中SYN表达上调,各干预组PSD-95蛋白表达均上调。与TP-L、TP-M及VE组相比,TP-H大鼠新异臂总路程增加;TP-H组突触间隙宽度较TP-L、TP-M组减小;TP-H组PSD厚度及数密度较其余干预组增加;与其余干预比,TP-H组SYN及PSD-95蛋白表达上调。结论TP可提高衰老合并雌激素缺乏大鼠记忆能力。其机制可能与TP干预可使衰老合并雌激素缺乏大鼠海马突触界面曲率增加,突触间隙变窄,PSD厚度及数密度增加,SYN和PSD-95表达上调,改善大鼠突触结构可塑性有关。
Objective To investigate the effects of tea polyphenols(TP)on memory and hippocampal synaptic structure in aging rats with estrogen deficiency.Methods Sixty SD female rats were randomly divided into the control group(Control,CN),the aging rats with estrogen deficiency group(Model,M),the TP-L,TP-L and TP-H treatment groups(75,150 and 300 mg/kg,respectively)and the VE group(60 mg/kg)(n=10).The CN rats were removed of an equal volume of adipose tissue near the ovary and were intraperitoneally injected with the same amount of normal saline,the model rats were induced by ovariectomy in combination with the injection of D-galactose[100 mg/(kg·d)].Every intervention group was intragastrically administered with the corresponding doses of TP.The CN and M groups were gavaged with the same amount of distilled water once a day for 12 weeks.Y maze test was used to measure the spatial memory.Transmission electron microscope was used to check the synaptic ultrastructure and the curvature of synaptic interface,synaptic cleft,PSD thickness and number density were analyzed quantitatively.SYN and PSD-95 protein expression in hippocampus was detected by Western blot.Results Compared with the CN group,the proportion of new alien arm entry times and total new alien arm distance decreased,synaptic interface curvature decreased,synaptic gap increased,PSD thickness and numerical densities decreased,and the protein expression of SYN and PSD-95 decreased in the M group.Compared with the M group,the proportion of new alien arm entry times increased in the TP-H and VE groups,and total new alien arm distance increased in all intervention groups.The synaptic interface curvature of the TP-H group was increased;the synaptic gap decreased and PSD thickness increased in the intervention groups;the numerical densities of the TP-M and TP-H groups increased.The expression of SYN was upregulated in the TP-M,TP-H and VE group.The expression of PSD-95 protein was upregulated in all intervention groups.Compared with the TP-L,TP-M and VE groups,the total new alien arm distance increased in the TP-H group.The synaptic gap of the TP-H group was smaller than that of the TP-L and TP-M groups.Compared with the other intervention groups,the PSD thickness and numerical densities increased in the TP-H group.The expression of SYN and PSD-95 in the TP-H group was up-regulated compared with other intervention groups.Conclusion TP intervention can improve the plasticity of synaptic structure in aging rats with estrogen deficiency.The mechanism may be related to increased curvature of hippocampal synaptic interface curvature,PSD thickness and numerical densities,decreased synaptic gap,up-regulated expression of SYN and PSD-95,whereby the plasticity of synaptic structure is improved in rats with aging and estrogen deficiency.
作者
石梦茜
宋晨萌
成乐
李学敏
张禄平
赵海峰
SHI Meng-qian;SONG Chen-meng;CHENG Le;LI Xue-min;ZHANG Lu-ping;ZHAO Hai-feng(Department of Nutrition and Food Hygiene,School of Public Health,Shanxi Medical University,Taiyuan 030001;Shanxi Provincial Center for Disease Control and Prevention,Taiyuan 030012,China)
出处
《营养学报》
CAS
CSCD
北大核心
2021年第5期468-473,共6页
Acta Nutrimenta Sinica
基金
国家自然科学基金(No.81973047,81472984)
关键词
茶多酚
衰老
雌激素缺乏
记忆减退
突触可塑性
大鼠
海马
tea polyphenols
aging
estrogen deficiency
memory impairment
synaptic plasticity
rat
hippocampus
作者简介
石梦茜(1995-),女,硕士研究生,E-mail:839344742@qq.com;通讯作者:赵海峰,E-mail:haifengzao75@163.com
