摘要
目的探讨长链非编码RNA(long non-coding RNA,LncRNA)HOX转录反义RNA(HOX transcript antisense RNA,HOTAIR)调控miR-148a-3p对乳腺癌细胞增殖、侵袭的影响。方法通过转染实验,将乳腺癌细胞T47D分为对照组、HOTAIR过表达组以及HOTAIR表达抑制组。采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRTPCR)方法检测3组细胞中HOTAIR与miR-148a-3p的表达情况;Cell Counting Kit-8(CCK-8)法及Transwell小室法检测各组转染细胞增殖、侵袭情况;双荧光素酶法检测HOTAIR与miR-148a-3p的靶向结合情况。结果qRT-PCR显示T47D细胞中,HOTAIR过表达与抑制效果显著(P<0.05)。与抑制组比较,过表达组T47D细胞中miR-148a-3p表达水平明显下降(P<0.05),细胞增殖及侵袭能力均升高(P<0.05)。野生型HOTAIR序列与miR-148a-3p模拟物共转染细胞的荧光素酶活性降低(P<0.05)。结论LncRNA-HOTAIR可通过miR-148a-3p影响T47D细胞的增殖、侵袭能力。
Objective To investigate the effects of long non-coding RNA(LncRNA)HOX transcript antisense RNA(HOTAIR)on the proliferation and invasion of breast cancer cells by targeting miR-148a-3p.Methods T47D cell line were divided into control group,HOTAIR overexpression group and HOTAIR inhibition group through transfection experiment.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of HOTAIR and miR-148a-3p in all 3 groups.Cell Counting Kit-8(CCK-8)and Transwell chamber method were used to detect the proliferation and invasion of transfected T47D cells.The targeted binding of HOTAIR to miR-148a-3p was detected by dual luciferase method.Results The qRT-PCR showed that overexpression and inhibition of HOTAIR were significant(P<0.05).Compared with the inhibition group,the expression level of miR-148a-3p in the overexpression group was significantly decreased(P<0.05),and cell proliferation and invasion ability were significantly increased(P<0.05).The luciferase activity of cells co-transfected with wild-type HOTAIR sequence and miR-148a-3p mimics was decreased(P<0.05).Conclusion LncRNA-HTOAIR may affect the proliferating and invading ability of T47D cells through miR-148a-3p.
作者
李子博
周江
周琳
李敏
何杰
龙丹
LI Zi Bo;ZHOU Jiang;ZHOU Lin;LI Min;HE Jie;LONG Dan(Department of Laboratory Medicine,Changsha Medical College,Changsha 410219,Hunan Province,China)
出处
《中国妇幼卫生杂志》
2022年第5期34-37,共4页
Chinese Journal of Women and Children Health
基金
2018年湖南省教育厅科学研究项目(18A499)
