摘要
目的探讨银屑病炎性微环境下,HaCaT细胞的糖代谢与增殖状况及两者的关系。方法将HaCaT细胞随机分为2组,采用M5(IL-1α、IL-17A、IL-22、抑癌蛋白M和TNF-α的混合物)和PBS缓冲液分别处理以模拟银屑病与正常人角质形成细胞(keratinocyte,KC)模型。分别采用细胞能量代谢分析仪检测细胞糖代谢水平、CCK-8检测细胞增殖、RT-qPCR检测K6、K16 mRNA相对表达量。结果与对照组相比,实验组糖酵解水平异常升高(P<0.01),有氧呼吸水平无明显变化(P>0.05);实验组A值及K6、K16的mRNA相对表达量均较对照组升高(P<0.05);相关性分析显示HaCaT细胞糖酵解水平与增殖存在正相关(P<0.05)。结论银屑病角质形成细胞模型的糖代谢模式表现为糖酵解的显著升高,其可能在调控银屑病角质形成细胞增殖方面发挥重要作用。
Objective To investigate the relationship between glucose metabolism and proliferation of HaCaT cells in the psoriatic microenvironment.Methods HaCaT cells were randomly divided into two groups,which were treated with M5(mixture of IL-1α,IL-17 A,IL-22,tumor suppressant protein M and TNF-α)and PBS buffer to simulate psoriasis and normal human keratinocyte(KC)model,respectively.Glucose metabolism,cell proliferation and mRNA expression levels of K6 and K16 were detected by the cell energy metabolism analyzer,CCK-8 and RT-qPCR,respectively.Results Compared with the control group,the level of glycolysis in the experimental group was much higher(P<0.01).There was no significant change in aerobic respiration(P>0.05).The A value and mRNA relative expression of K6 and K16 in the experimental group were higher than in the control group(all P<0.05).Correlation analysis showed that the glycolysis level of HaCaT cells was positively correlated with proliferation(P<0.05).Conclusion The glucose metabolism pattern of the psoriatic KC model is marked by significantly increased glycolysis,which may play an important role in regulating the proliferation of Psoriatic KC.
作者
成彦蓉
李俊琴
杨艳妮
杨旭燕
李新华
CHENG Yanrong;LI Junqin;YANG Yanni;YANG Xuyan;LI Xinhua(Department of Dermatology,the Ninth Clinical Medical College of Shanxi Medical University,Taiyuan 030000,China)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2022年第10期1132-1136,共5页
The Chinese Journal of Dermatovenereology
基金
山西省医学重点科研项目(2020XM20)
作者简介
通信作者:李新华,E⁃mail:tylixinhua@sina.com
