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弹力纤维醛品红法过染时解决方法的探讨

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摘要 弹力纤维广泛分布于身体各处,在HE染色中呈淡红色,弹力纤维主要染色方法有Gomori醛品红法、Weigert间苯二酚碱性品红法、维多利亚蓝法等[1]。本科室主要采用Gomori醛品红法,笔者发现在醛品红染色结束时如果先用水冲掉醛品红染液再用70%乙醇分化,乙醇的分化作用会显著减弱,导致切片过染,背景过度着色,整个视野一片深紫色(图1),且不能再被乙醇洗脱,背景过深,影响诊断。鉴于此,笔者通过实践和摸索,依据酸碱度分三种类型进行试验分别比较过染褪色和褪色后再次染色的效果,现介绍如下。
出处 《诊断病理学杂志》 2023年第8期819-820,共2页 Chinese Journal of Diagnostic Pathology
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