摘要
采用分子生物学方法、电化学检测方法与表面形貌观察等手段研究了环境友好型的D-tyrosine在抑制和分散腐蚀性铁细菌生物膜菌落中的杀菌增强效果和作用机理。结果表明:D-tyrosine能分解试样表面上的生物膜,使其由附着态转变为浮游态,进入水体,被水中的杀菌剂快速杀灭,进而抑制微生物腐蚀的发生;Dtyrosine+杀菌剂的杀菌效果十分显著,杀菌率可达98.73%,明显优于单加杀菌剂的杀菌效果,且杀菌剂的使用量下降50%~70%。表面分析结果表明,溶液中加入杀菌剂和D-tyrosine后,可使附着在试样表面上的IOB膜快速分解脱落,有效地减缓了腐蚀的发生;腐蚀产物均以Fe的氧化物为主。阻抗谱分析表明,添加D-tyrosine+杀菌剂组合后试样的极化电阻增大,腐蚀速率降低。分析表明,D-tyrosine对杀菌剂的增强效果十分明显,对微生物腐蚀起到很好的抑制减缓作用,有效地缓解了过量杀菌剂引起的微生物抗药性及环境污染问题。
In order to solve the problem of serious environmental pollution caused by traditional chemical sterilization methods,the bactericidal enhancement effect and relevant mechanism of D-tyrosine in inhibiting and dispersing corrosive iron bacteria biofilm were studied by means of molecular biology,electrochemical detection and surface morphology observation.The results showed that D-tyrosine can successfully disperse the biofilm on the surface of the sample,turn which from adherent state to planktonic state entering the water system,thus inhibit the occurrence of microbial corrosion,while the bacteria of the planktonic iron bacteria film can be quickly killed by bactericide in the water.The bactericidal effect of the combined D-tyrosine and bactericide is very remarkable,the bactericidal rate can reach98.73%,which is better than that of single bactericide,therefore,the dosage of bactericide can be decreased by 50%~70%.The results of surface analysis showed that the corrosion products were mainly iron oxides when bactericides and D-tyrosine were added to the solution,and the product film formed on the sample surface was loose and easy to fall off,which effectively slowed down the corrosion.The impedance spectroscopy showed that the polarization resistance of the sample increased and the corrosion rate decreased after adding D-tyrosine acids and bactericides.The above analysis showed that D-tyrosine had obvious enhancement effect on bactericides,played a very good role in inhibiting the microbial corrosion,and effectively alleviated the problems of microbial drug resistance and environmental pollution caused by the application of excessive microbicides.
作者
胥聪敏
罗立辉
王文渊
赵苗苗
田永强
宋鹏迪
XU Congmin;LUO Lihui;WANGWenyuan;ZHAO Miaomiao;TIAN Yongqiang;SONG Pengdi(School of Materials Science and Engineering,Xi'an Shiyou University,Xi'an 710065,China)
出处
《中国腐蚀与防护学报》
CAS
CSCD
北大核心
2020年第1期63-69,共7页
Journal of Chinese Society For Corrosion and Protection
基金
国家自然科学基金(21808182)
中国石油科技创新基金(2018D-5007-0216)
陕西省重点学科专项(YS37020203)
陕西省能源化工过程强化重点实验室项目(SXECPI201503)
西安石油大学研究生创新与实践能力培养项目(YCS17111007和YCS19113064).
关键词
微生物腐蚀
D-tyrosine
铁细菌
杀菌剂
杀菌增强效果
microbiologically influenced corrosion
D-tyrosine
iron bacteria
bactericide
bactericidal enhancement effect
作者简介
胥聪敏,女,1977年生,博士,副教授;通讯作者:胥聪敏,E-mail:cmxu@xsyu.edu.cn,研究方向为金属腐蚀与防护
