摘要
为了探究鹅源产气荚膜梭菌(Clostridium perfringens,Cp)的生物学特性,从临床送诊的病死鹅肠道分离出4株疑似产气荚膜梭菌,菌株纯化后分别进行革兰染色、生化鉴定、16 S rDNA序列分析、分子分型和药敏试验。结果显示:4株分离菌为革兰阳性菌,仅在厌氧条件下生长,且出现典型双溶血环菌落,16 S rDNA鉴定为产气荚膜梭菌,分别命名Cp1、Cp2、Cp3和Cp4;经多重PCR鉴定,4株菌仅含α毒素,无其他毒素,故属于A型产气荚膜梭菌;药敏结果显示4株菌对链霉素、庆大霉素、复方新诺明、多黏菌素B等多种抗生素耐药,对阿莫西林、头孢曲松钠、环丙沙星、氟苯尼考等敏感。综上所述,从临床病鹅分离到4株A型Cp,为鹅源Cp的生物特性研究提供了参考材料。
In order to explore the biological characteristics of Clostridium perfringens(Cp)from goose,four suspected Cp were isolated from intestines of dead geese.The isolates were purified,and carried on Gram staining,biochemical test,16 S rDNA gene sequence analysis,molecular typing and drug sensitivity test,respectively.The results showed that the four isolates were Gram-positive,grew only under anaerobic conditions,appeared typical double hemolytic ring colonies,and were identified as Cp by 16 S rDNA gene sequence analysis.The four strains were named by Cp1,Cp2,Cp3 and Cp4.All of the four isolated strains were identified as Cp type A in term of only containingαtoxin by a multiplex PCR.The results of drug sensitivity indicated that four strains were resistant to more than three antibiotics such as streptomycin,gentamycin,co-trimoxazole,polymyxin B.Four strains were sensitive to amoxicillin,ceftriaxone sodium and florfenicol.Therefore,the four Cp type A provided scientific basis for the study of biological characteristics of Cp in goose.
作者
候照峰
冯茜茜
李贝贝
孙启亮
丁志刚
陈凌志
王丽扬
魏诗瑶
张信军
许金俊
潘志明
陶建平
王彦红
HOU Zhaofeng;FENG Qianqian;LI Beibei;SUN Qiliang;DING Zhigang;CHEN Lingzhi;WANG Liyang;WEI Shiyao;ZHANG Xinjun;XU Jinjun;PAN Zhiming;TAO Jianping;WANG Yanhong(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009;Jiangsu Key Laboratory of Zoonosis,Yangzhou,Jiangsu 225009;Heze Academy for Food and Drug Control,Heze,Shandong 274000)
出处
《中国家禽》
北大核心
2022年第5期32-37,共6页
China Poultry
基金
国家自然科学基金(32002303)
江苏省基础研究计划(BK20190885)
中国博士后科学基金(2020M671615)
扬州大学大学生创新创业计划项目(X20210704、X20210701)
江苏省人兽共患病学重点实验室资助项目(R1912)
江苏省高校优势学科建设工程项目
关键词
鹅
产气荚膜梭菌
生化试验
16
S
rDNA鉴定
分子分型
药敏试验
goose
Clostridium perfringens
biochemical test
16 S rDNA identification
molecular typing
drug sensitivity test
作者简介
候照峰(1987-),男,博士,讲师,主要从事动物疫病诊断与防控技术研究,E-mail:zfhou@yzu.edu.cn;冯茜茜(1998-),女,硕士研究生,研究方向为预防兽医学,E-mail:1411064524@qq.com;通讯作者:王彦红(1974-),女,博士,讲师,主要从事动物疫病诊断与防控技术研究,E-mail:wyh7405@163.com;同等贡献:候照峰;同等贡献:冯茜茜
