摘要
目的探讨高尿酸血症鹌鹑肠道菌群结构变化特点,为高尿酸血症病理机制研究提供参考。方法将迪法克鹌鹑随机分为两组:正常组和模型组,正常组喂饲正常饲料,模型组喂饲高嘌呤饲料;PCR-DGGE法构建两组鹌鹑盲肠内容物菌群DNA指纹图谱,对图谱进行数字化处理,聚类分析法比较两组鹌鹑肠道菌群结构整体差异,logistic回归法辨别差异条带,克隆和鉴定差异条带序列;HE染色观察盲肠组织结构变化。结果造模第28天,模型组鹌鹑与正常组比较,血尿酸显著升高、肠道菌群整体结构发生变化,DGGE指纹图谱上12条差异条带,序列鉴定归属于拟杆菌门、厚壁菌门、变形菌门;盲肠组织出现炎性变化。结论高嘌呤饮食诱导的高尿酸血症鹌鹑肠道菌群结构发生改变。
Objective To investigate the gut microbiota changes in quails with hyperuricemia and provide a reference for the pathological mechanism of hyperuricemia.Methods Difalk quails were randomly divided into the normal and model groups and fed a normal diet or high-purine diet,respectively.DNA fingerprinting of the intestinal flora in the cecal contents of quails was performed using the PCR-DGGE method and digitized to analyze the gut microbiota structure via clustering analysis.Logistic regression was used to discern the different bands,and the genes of the different bands were cloned and identified.The cecal tissue was observed via hematoxylin and eosin staining.Results The gut microbiota structure of the model group quails differed from that of the normal group quails on day 28 of the experiment.The DGGE fingerprint contained 12 bands,belonging to Bacteroidetes,Firmicutes,and Proteobacteria.Inflammatory changes occurred in the cecum.Conclusions The gut microbiota structure is altered in quails with high-purine diet-induced hyperuricemia.
作者
黄胜男
林志健
张冰
吕锦涛
王如然
HUANG Shengnan;LIN Zhijian;ZHANG Bing;LYU Jintao;WANG Ruran(South of Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 102618,China;Beijing University of Chinese Medicine,Beijing 100029)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2020年第1期17-22,共6页
Acta Laboratorium Animalis Scientia Sinica
基金
国家自然科学基金资助项目(81673618)
国家中医药领军人才支持计划-“岐黄学者”支持项目
中国中医科学院广安门医院(南区)科研基金(Y2017-20).
作者简介
黄胜男(1980—),女,副主任药师,博士研究生,研究方向:代谢性疾病中医药防治研究。Email:huangsn1112@126.com;通信作者:张冰(1959—),女,主任医师,教授,博士生导师,研究方向:代谢性疾病中医药防治研究。Email:zhangbin6@263.net
