摘要
目的探讨反流性食管炎(RE)患者口腔菌群变化及其与幽门螺杆菌(H.pylori)感染的关系,为该类患者的治疗提供参考。方法选择南京同仁医院2019年5月至2021年5月收治的140例RE患者为研究对象,将A、B级RE患者纳入AB组,C、D级纳入CD组;同时将患者分为合并H.pylori感染组和未合并H.pylori感染组。以一次性无菌收集管收集患者唾液标本,使用Illumina Hiseq 2500高通量测序平台对合格文库进行双端测序。结果AB组患者H.pylori阳性率(16.67%)低于CD组(45.45%)。AB组患者口腔菌群Chao 1指数和Shannon指数均高于CD组,Simpson指数低于CD组(均P<0.05)。未合并H.pylori感染组患者口腔菌群Chao 1指数和Simpson指数高于合并H.pylori感染组,Shannon指数低于合并H.pylori感染组(均P<0.05)。未合并H.pylori感染组患者口腔放线菌门、放线菌属相对丰度高于合并H.pylori感染组,拟杆菌门相对丰度低于合并H.pylori感染组(均P<0.05)。结论RE病情较重者和合并H.pylori感染者表现为口腔菌群α多样性降低和门、属水平菌群相对丰度改变,合并H.pylori感染对RE患者病情和口腔菌群会产生一定影响。
Objective To observe the changes of oral microecology in patients with reflux esophagitis(RE)and its relationship with Helicobacter pylori(H.pylori)infection.Methods A total of 140 RE patients admitted to Nanjing Tongren Hospital from May 2019 to May 2021 were selected as the subjects.The RE patients with grades A and B were included in the AB group,while those with grades C and D were included in the CD group.Upon recombination,the patients were divided into H.pylori infection group and non-H.pylori infection group.Saliva samples were collected in disposable sterile collection tubes.Eligible libraries were paired-end sequenced using the Illumina Hiseq 2500 high-throughput sequencing platform.Results The positive rate of H.pylori in AB group was lower than that in CD group(16.67%vs 45.45%).The oral flora Chao 1 index and Shannon index in the AB group were higher,while the Simpson index was lower than in the CD group,respectively(all P<0.05).The oral flora Chao 1 index and Simpson index in non-H.pylori group were higher,while the Shannon index was lower than in H.pylori infection group,respectively.The relative abundances of Actinobacteria and Actinomyces in non-H.pylori group were higher,while that of Bacteroidetes was lower than in the H.pylori infection group respectively(all P<0.05).Conlclusion Severe RE patients and patients with H.pylori infection showed a decrease in theαdiversity of oral colonies and changes in the relative abundance at phylum and genus levels.Concommitant H.pylori infection had a certain impact on RE disease and oral microecological communities.
作者
王利丽
李兰花
李文伦
栗欣
李艳慧
WANG Li-li;LI Lan-hua;LI Wen-lun;LI Xin;LI Yan-hui(Department of Gastroenterology,Nanjing Tongren Hospital,Nanjing,Jiangsu 211102,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2022年第12期1432-1435,共4页
Chinese Journal of Microecology
关键词
反流性食管炎
口腔菌群
幽门螺杆菌
Α多样性
相对丰度
Reflux esophagitis
Oral microecology
Helicobacter pylori
Alpha diversity
Relative abundance
作者简介
通信作者:王利丽(1985-),女,本科,从事临床内镜检测,E-mail:shuyyone@163.com.
