摘要
代谢组学是系统生物学的分支学科,在诊断标志物的发现、疾病预后转归、药物作用机制、药效评价、药物毒性评估、中医证型判断等领域得到了广泛应用,传统代谢组学研究存在若干不足之处。微透析技术是新型生物采样技术,基于微透析技术的代谢组学研究方兴未艾。鉴于微透析技术的特殊性及其与传统采样、前处理方法的巨大差异性,基于微透析技术的代谢组学流程与传统的代谢组学研究相比既具有一定相似性,又具有特殊性。该文通过系统总结微透析技术进行代谢组学研究的优势、基本策略等,以期为研究人员借鉴。
Metabonomics is the branch of systems biology.It has been widely used in the fields of diagnostic markers discovery,disease prognosis,drug action mechanism,drug efficacy and toxicity evaluation,traditional Chinese medicine syndromes differentiation.There are shortcomings in the conventional metabonomics research.Microdialysis technology is a new type of biosampling technology,and metabonomics research based on microdialysis technology is in the ascendant.In view of the particularity of microdialysis technology and its great differences from traditional sampling and pretreatment methods,the metabonomics process based on microdialysis technology has certain similarities with traditional metabonomics research,and its basic process has some particularity.Advantages and basic strategies of metabonomics research by microdialysis technology are systematically summarized for researchers′reference.
作者
张英丰
黄星星
朱黎霞
ZHANG Ying-feng;HUANG Xing-xing;ZHU Li-xia(College of Chinese Materia Medica,Guangzhou University of Traditional Chinese Medicine,Guangzhou 510006,China;Department of Traditional Chinese Medicine,Zhujiang Hospital,South Medical University,Guangzhou 510282,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第1期214-220,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81673889,81873227)
广东省科技计划项目(2014A020221102).
关键词
微透析技术
代谢组学
研究策略
探讨
microdialysis technique
metabonomics
research strategy
discussion
作者简介
张英丰,副教授,E-mail:zyfeng-2006@163.com;通信作者:朱黎霞,副主任医师,E-mail:zhulx2007@163.com
