摘要
探讨黄芩清热除痹胶囊(HQC)对强直性脊柱炎(AS)患者疗效及氧化应激的影响,探究其可能机制。选取58例AS患者随机分为HQC组与柳氮磺吡啶(SASP)组,另设30例健康对照组。采用ELISA检测血清超氧化物歧化酶(SOD)、总抗氧化能力(TAOC)、过氧化脂质(LPO)、丙二醛(MDA)、白介素(IL)1β、IL-10、IL-4、肿瘤坏死因子-α(TNF-α)水平;运用实时荧光定量PCR(RT-qPCR)检测腺苷酸活化蛋白激酶(AMPK-α)、叉头转录因子O3a(FOXO3a)、锰超氧化物岐化酶(MnSOD)、过氧化体增殖物激活型受体γ(PPARγ)mRNA表达;采用蛋白免疫印迹法(Western blot)检测AMPK-α,FOXO3a,p-FOXO3a,MnSOD,PPARγ蛋白表达;采用问卷调查评价疾病活动评分并观察HQC对AS患者临床疗效。AS患者外周血中MDA,LPO,TNF-α,IL-1β水平升高,SOD,TAOC,IL-4,IL-10水平降低;HQC治疗后疾病活动评分均显著降低,疗效显著高于SASP组;HQC治疗后TAOC,SOD,IL-4,IL-10显著升高,MDA,LPO,TNF-α,IL-1β显著下降;HQC治疗后AMPK-α,FOXO3a,MnSOD,PPARγmRNA及AMPK-α,FOXO3a,p-FOXO3a,MnSOD,PPARγ蛋白表达显著升高(P<0.01或P<0.05)。结果表明HQC可改善AS患者临床症状及氧化应激,其机制可能与激活PPARγ上调AMPK/FOXO3a信号通路有关。
To investigate the efficacy of Huangqin Qingre Chubi Capsules(HQC)in patients with ankylosing spondylitis(AS)and its effect on oxidative stress,and to explore its possible mechanism.Fifty-eight cases of AS patients were randomly divided into HQC group and salazosulfapyridine(SASP)group.Another 30 healthy people were employed as a control group.Superoxide dismutase(SOD),total antioxidant capacity(TAOC),malondialdehyde(MDA),lipid peroxidatio(LPO),interleukin-1β(IL-1β),IL-10,IL-4,and tumor necrosis factor-α(TNF-α)were detected by ELISA.The mRNA expression levels of AMP-activated protein kinase(AMPK-α),forkhead box O3a(FOXO3a),manganese superoxide dismutase(MnSOD),and peroxisome proliferator-activated receptor gamma(PPARγ)were detected by Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).The protein expression levels of AMPK-α,FOXO3a,p-FOXO3a,MnSOD,and PPARγwere detected by Western blot.A questionnaire was used to evaluate the disease activity score and observe the clinical efficacy of HQC in AS patients.The levels of MDA,LPO,TNF-α,and IL-1βwere significantly increased in the peripheral blood of AS patients,and SOD,TAOC,IL-4,IL-10 levels were significantly decreased.After HQC treatment,scores of disease active indexes were all decreased,and its clinical efficacy was significantly higher than that in SASP group.After HQC treatment,TAOC,SOD,IL-4,IL-10 were increased and MDA,LPO,TNF-α,IL-1βwere decreased;mRNA levels of AMPK-α,FOXO3a,MnSOD,PPARγand protein levels of AMPK-α,FOXO3a,p-FOXO3a,MnSOD,PPARγwere increased(P<0.01 or P<0.05).HQC can effectively improve the clinical symptoms and oxidative stress of AS patients,and its mechanism may be related to activating PPARγand up-regulating AMPK/FOXO3a signal pathway.
作者
黄旦
刘健
纵瑞凯
万磊
HUANG Dan;LIU Jian;ZONG Rui-kai;WAN Lei(Anhui University of Traditional Chinese Medicine,Hefei 230038,China;Department of Rheumatology,First Affiliated Hospital,Anhui University of Traditional Chinese Medicine,Hefei 230031,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第2期451-456,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81503558)
安徽省科技攻关项目(1604a0802085)
安徽省重点实验室建设项目(1306c083035)
2016年中央引导地方科技发展专项(财教[2016]1188).
作者简介
黄旦,博士研究生,E-mail:1419865742@qq.com;通信作者:刘健,教授,主任医师,博士生导师,研究方向为中医药防治风湿病,E-mail:liujianahzy@126.com
