摘要
壮观放线菌素产生菌Streptomycesspectabilis 2233经紫外线诱变获得的营养缺陷型菌株SS 612(thr~-)和SS 624(his~-),采用溶菌酶脱壁形成原生质体后,进行种内融合。以营养缺陷作为选择标记,获得了大量异养型及原养型重组子。融合频率达15%。重组子遗传分析表明,异养型和原养型重组子数量大致相等。
Two auxotrophic strains,SS612(thr)andSS624(his),were obtained from Streptomycesspectabilis 2233 by U.V.induction.Their pro-toplasts were prepared by enzymatic digestionof the cell wall with lysozyme,and then trea-ted with polyethylene glycol for intraspecificfusion.Recombinants were isolated on selectivemedia.Recombination frequency of about 15%was observed.Genetic analysis indicated thatthe number of double auxotrophic recombinantwas nearlyi equal to that of prototrophic recom-binant,and another marker,orn,different fromthe two parentals was observed in the doubleauxotrophic recombinants.Other analysesshowed that the antibacterial spectrum of re-combinant FA40 differed from the parentals,and a new component appeared on the paperchromatography of recombinan FA8.Thissuggested that silent gene might be activatedafter recombination.In shaking flask fermenta-tion,the potency of recombinant FP221 increased56% as compared with those of the originalstrain and the intermediate parents.
出处
《医药工业》
CAS
1987年第1期28-28,共1页
