摘要
目的对核酸检测及ELISA检测的效果予以分析评估,以使血液传播疾病的发生率降低。方法随机选取2017年1月至2019年12月间100份抗-HIV、抗-HCV及HBsAg灰区标本和反应性标本实施ELISA检测,对比3000份同期实施核酸检测的ELISA阴性标本检验结果。结果对比核酸检测结果,实施单阳及双阳试剂ELISA检测的100份标本结果与其存在差异,HBsAg检测的灰区标本有一定检出率,其他检测无反应。ELISA阴性标本3000份检出有反应性的4份,HBV DNA存在反应性的有1份,阴性为1份。结论对于实施ELISA检测的标本,若结果为阴性还需实施核酸检测,窗口期感染率和灰区值之间的关系需更深层次的分析。若与核酸检测结果相比,ELISA灰区及反应性标本结果存在差异,核酸为阳性而HBsAg灰区或阴性的患者,要鉴别区分其是假阳性、隐匿性感染还是窗口期,需通过跟踪随访予以了解。
Objective to analyze and evaluate effect of nucleic acid detection and ELISA detection,and reduce incidence of blood borne diseases.Methods The paper chose 100 anti HIV,anti HCV and HBsAg gray area samples and reactive samples from January 2017 to December 2019 randomLy for ELISA detection,compared 3000 negative samples of nucleic acid detection at the same period.Results:Nucleic acid detection comparison showed,detection results of 100 samples had difference between single positive and double positive ELISA test.Gray area samples detected by HBsAg had some certain detection rate,and other tests had no reaction.Among 3000 ELISA negative samples,4 were found to be reactive,1 was HBV DNA reactive and 1 was negative.Conclusion For samples tested by ELISA,if result is negative,nucleic acid test should be carried out.Relationship between window period infection rate and gray area value needs further analysis.If there are differences between results of ELISA gray area,reactive samples and those of nucleic acid test,patients with positive nucleic acid and negative HBsAg gray area should be identified as false-positive,occult infection or window period by follow-up.
作者
王梅瑛
WANG Mei-ying(Quality Control Department,Haixi Prefecture Central Blood Station,Haixi,Qinghai,817099)
出处
《智慧健康》
2021年第18期31-33,共3页
Smart Healthcare
关键词
ELISA反应性
灰区
阴性标本
核酸检测
ELISA reactivity
Grey area
Negative specimens
Nucleic acid detection
