摘要
目的 探讨天麻素(Gas)对呼吸道合胞病毒(RSV)感染肺炎大鼠肺组织的保护机制及对Th17/Treg细胞平衡的影响。方法 将Wistar雄性大鼠60只分为对照组(C组)、模型组(M组)Gas低剂量组(Gas-L,5 mg/kg)、Gas中剂量组(Gas-M,10 mg/kg)、Gas高剂量组(Gas-H,20 mg/kg)、阳性组(0.15 g/kg利巴韦林),每组10只。药物干预结束后,称定大鼠体质量和肺组织干湿重,计算肺指数和肺组织W/D值;取肺组织作HE染色检查并进行损伤评分;qRT-PCR检测肺组织RSV病毒载量;流式细胞术检测脾组织Th17、Treg细胞比例;ELISA法检测血清TNF-α、IL-1β、IL-6、IL-17A、TGF-β含量;Western blot检测肺组织Notch/NF-κB通路蛋白表达。结果 与C组相比,M组肺组织出现中性粒细胞浸润,肺泡破裂连片等病变,肺指数、W/D值、肺损伤评分、RSV病毒载量、Th17细胞比例、TNF-α、IL-1β、IL-6、IL-17水平、Notch-1、Hes-1、p-NF-κB p65/NF-κB p65蛋白均显著升高(均P<0.05),Treg细胞比例和TGF-β水平均显著下降(均P<0.05)。Gas-M、H组和阳性组肺损伤程度显著改善,肺指数、W/D值、肺损伤评分、RSV病毒载量,Th17细胞比例,TNF-α、IL-1β、IL-6、IL-17及Notch-1、Hes-1、p-NF-κB p65/NF-κB p65蛋白水平均显著下降(均P<0.05),Treg细胞比例和TGF-β水平显著上升(均P<0.05)。结论 Gas具有减轻RSV感染肺炎大鼠的肺组织炎症损伤和维持Th17/Treg细胞平衡的作用,可能通过抑制Notch/NF-κB信号通路实现。
Objective To investigate the protective mechanism of gastrodin(Gas) on lung tissue of rats with respiratory syncytial virus(RSV)-infected pneumonia and its influence on Th17/Treg cell balance. Methods Sixty Wistar male rats were grouped into control group(C group),model group(M group),Gas low-dose group(Gas-L,5 mg/kg),Gas medium-dose group(Gas-H,10 mg/kg),Gas high-dose group(Gas-M,20 mg/kg),and positive group(0.15 g/kg ribavirin),10 in each group. After the drug intervention, the body weight and the dry and wet weight of the lung tissue were weighed, and the lung index and the W/D value of the lung tissue were calculated;lung tissue was stained with HE and scored for damage;qRT-PCR was applied to detect RSV viral load in lung tissue;flow cytometry was applied to detect the proportions of Th17 and Treg cells in spleen tissue;ELISA method was applied to detect serum levels of TNF-α,IL-1β,IL-6,IL-17A,TGF-β;Western Blot was applied to detect Notch/NF-κB pathway protein expression in lung tissue. Results Compared with group C,the lung tissue of M group showed neutrophil infiltration, alveolar rupture and contiguous lesions, the lung index, W/D value, lung injury score, RSV viral load, the proportion of Th17 cells, levels of TNF-α,IL-1β,IL-6,IL-17,and the protein levels of Notch-1,Hes-1,and p-NF-κB p65/NF-κB p65 increased significantly(P<0.05),and the proportion of Treg cells and the level of TGF-β decreased obviously(P<0.05). The degree of lung injury in the Gas-M、H groups and the positive group was obviously improved, the lung index, W/D value, lung injury score, RSV viral load, the proportion of Th17 cells, levels of TNF-α,IL-1β,IL-6,IL-17,and the protein levels of Notch-1,Hes-1,and p-NF-κB p65/NF-κB p65 decreased obviously(P<0.05),and the proportion of Treg cells and the level of TGF-β increased obviously(P<0.05). Conclusion Gas can reduce the inflammatory injury of lung tissue and maintain the balance of Th17/Treg cells in RSV-infected pneumonia rats, which may be achieved by inhibiting Notch/NF-κB signaling pathway.
作者
冷晓雪
李柏新
陈之光
LENG Xiao-xue;LI Bai-xin;CHEN Zhi-guang(Department of Emergency,Shengjing Hospital Affiliated to China Medical University,Shenyang 110100,China;Rehabilitation Center,Shengjing Hospital Affiliated to China Medical University)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第1期6-11,共6页
Journal of Pathogen Biology
作者简介
冷晓雪(1984-),女,吉林磐石人,大专,护师,主要从事呼吸机相关肺炎,感染,脓毒症等方面的研究。E-mail:lengxiaoxue8886@163.com;通讯作者:李柏新,E-mail:liboxin8880@163.com
