摘要
为了研究菜豆种质中植物凝集素的遗传多样性及含量变化,本研究以317份菜豆品种为试验材料,利用改进的血凝法测定鲜荚中的植物凝集素含量。同时,对不同发育时期‘紫冠’和‘白珍珠’的豆荚、荚壁和种子中的植物凝集素含量进行测定分析。结果表明:菜豆种质中植物凝集素含量变幅为0.004~1.783 mg/g,变异系数为0.493,品种频率分布曲线总体显现连续性,主峰明显,呈近似正态分布。菜豆品种群体间的凝集素水平差异极显著,表现出明显的种质特异性和遗传多样性‘。紫冠’和‘白珍珠’豆荚在花后4~6天中检测到少量凝集素,表明豆荚凝集素是在花后4天开始积累。‘紫冠’与‘白珍珠’的植物凝集素含量随着豆荚生长发育都呈现出相同趋势,先上升后下降,再上升后下降,出现‘双峰’现象。
To study the genetic diversity and content variation of phytohemagglutinin(PHA) in common bean germplasm, 317 bean varieties were used to determine plant PHA in fresh pods by improved hemagglutination method. PHA contents in‘Purple Crown’and‘White Pearl’in different development stages of pods, pod walls and seeds were also measured. The results showed that the PHA content in common bean was 0.004-1.783 mg/g, and the coefficient of variation was 0.493. The frequency distribution curve was continuous and the main peak was obvious, showing an approximate normal distribution. PHA levels had significant differences among common bean populations, which showed obvious germplasm specificity and genetic diversity. Low PHA level was detected in‘Purple Crown’and‘White Pearl’pods from 4 to 6 days after flowering, indicating that the PHA in pod began to accumulate in the fourth day after flowering. The PHA of‘Purple Crown’and‘White Pearl’had the same trend with the growth and development of pods, first rose and then decreased, and then rose and decreased again, had the phenomenon of‘double peaks’.
作者
胡燕秋
冯国军
杨晓旭
刘畅
刘大军
闫志山
Hu Yanqiu;Feng Guojun;Yang Xiaoxu;Liu Chang;Liu Dajun;Yan Zhishan(College of Life Science,Heilongjiang University,Harbin 150080;Crop Research Institute of Heilongjiang University,Harbin 150080)
出处
《中国农学通报》
2020年第5期18-25,共8页
Chinese Agricultural Science Bulletin
基金
中国博士后基金面上项目“菜豆凝集素含量主效QTL精细定位及候选基因分析”(2018M631973)
黑龙江省应用技术研究与开发计划重大项目“寒地果菜优质高效栽培技术研究与示范”(GY2019YF0059)
省自然科学基金联合引导项目“菜豆荚型主效基因的克隆与功能基因解析”(LH2019C058)
黑龙江省高校基本科研业务(KJCXYB201707).
关键词
菜豆
植物凝集素
血凝法
遗传多样性
凝集素含量动态变化
common bean
PHA
hemagglutination assay
genetic diversity
dynamic change of lectin content
作者简介
第一作者:胡燕秋,女,1995 年出生,四川乐山人,硕士,研究方向:蔬菜种质资源创新及利用。通信地址:150080 黑龙江省哈尔滨市南岗区黑龙江大学农学楼,E-mail:smile_hyq@163.com;通讯作者:冯国军,男,1966 年出生,黑龙江克山人,研究员,博士,研究方向:蔬菜种质资源创新及利用。通信地址:150080 黑龙江省哈尔滨市南岗区黑龙江大学农学楼,Tel:0451-86604982,E-mail:feng998@126.com。
