摘要
研究旨在筛选出优良抗旱育种材料,为今后甘蔗抗旱育种和抗旱栽培技术提供重要的理论依据。采用盆栽干旱处理,测定蔗茅野生种无性系、甘蔗栽培品种(系)及其杂交后代共12份材料苗期与抗旱相关的8项生理生化指标,利用极点排序法对供试材料的抗旱性进行了综合评价。结果表明:干旱胁迫下,12份供试材料的Pro、MDA、SS及PMP均呈上升趋势,SP、Chl和SOD活性呈下降趋势,CAT活性有升有降。极点排序法分析表明,12个供试材料的抗旱强弱为:’蔗茅99-2’>’蔗茅99-1’>’蔗茅99-4’>’蔗茅I91-8’>’蔗茅99-3’>’蔗茅90-29’>’滇蔗01-58’>’滇蔗04-14’>’滇蔗02-39’>’蔗茅II91-2’>’新台糖10’>’崖城89-9’,说明蔗茅优质抗旱性状可以通过基因渗透方式传递给后代。在苗期干旱胁迫下’,蔗茅99-2’在渗透调节等方面表现出积极的生理响应。
The purpose of this study is to screen out excellent drought-resistant breeding materials, and to provide a basis for drought-resistant breeding and drought-resistant cultivation techniques of sugarcane. A total of 12 materials of Saccharum spp × Erianthus fulvus and their hybrid offspring were treated with pot drought treatment at seedling stage. Eight physiological and biochemical indexes related to drought resistance were tested. The drought resistance of the tested materials was evaluated comprehensively by pole-sorting method. The results showed that the indexes of Pro, MDA, SS and PMP of the 12 tested materials had an upward trend, the indexes of SP, Chl and SOD had a downward trend, while the CAT activity increased or decreased under drought stress. The results of pole-sorting method analysis showed that the drought resistance of the 12 tested materials was as follows:’ZM99-2’>’ZM99-1’>’ZM99-4’>’ZMI91-8’>’ZM99-3’>’ZM90-29’>’DZ01-58’>’DZ04-14’>’DZ02-39’>’ZMII91-2’>’ROC10’>’YC89-9’. It indicated that the high quality drought resistance traits of Erianthus fulvus could be transmitted to offspring by gene penetration.’ZM99-2’showed positive physiological response in osmotic adjustment and other aspects at seedling stage under drought stress.
作者
沈先岳
徐荣
吴清莲
谢林艳
孟玉
狄义宁
王先宏
何丽莲
李富生
Shen Xianyue;Xu Rong;Wu Qinglian;Xie Linyan;Meng Yu;Di Yining;Wang Xianhong;He Lilian;Li Fusheng(College of Agronomy and Biotechnology,Yunnan Agricultural University,Kunming 650201;Sugarcane Research Institute,Yunnan Agricultural University,Kunming 650201)
出处
《中国农学通报》
2020年第20期7-13,共7页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目“基于转录组测序的甘蔗野生种抗逆性相关基因克隆与分析”(31560417)
云南省应用基础研究计划重点项目“基于蔗茅资源利用的抗旱耐寒甘蔗育种基础研究”(2015FA024)
关键词
甘蔗
蔗茅
生理指标
干旱胁迫
极点排序法
sugarcane
Erianthus fulvus
physiological indexes
drought stress
pole-sorting method
作者简介
第一作者简介:沈先岳,男,1994 年出生,安徽芜湖人,在读研究生,硕士,研究方向:甘蔗资源研究与利用方面的研究。通信地址:650201 云南省昆明市盘龙区云南农业大学农学与生物技术学院,E-mail:925628070@qq.com。;通讯作者:何丽莲,女,1970 年生,四川洪雅人,高级实验师,硕士,主要从事甘蔗资源研究与利用方面的研究。通信地址:650201 云南省昆明市盘龙区云南农业大学农学与生物技术学院,E-mail:helilian905@sohu.com。;通讯作者:李富生,男,1968 年生,河南确山人,教授,博士,主要从事甘蔗资源研究与利用。通信地址:650201 云南省昆明市盘龙区云南农业大学农学与生物技术学院,Tel:13888191720,E-mail:Lfs810@sina.com。
