摘要
为了寻找耐盐新基因,培育耐盐新品种。本研究以23个小麦品种为材料,对TaPLD-2基因进行克隆及序列多态性分析,并对TaPLD-2基因响应盐胁迫表达模式进行了研究。结果表明,TaPLD-2基因的全长编码框为1301 bp,位于2DS染色体上,编码的蛋白定位于细胞质,为亲水蛋白,无跨膜区域,属于可溶性NAD(P)(H)氧化还原酶家族;TaPLD-2基因受盐胁迫诱导上调表达,表达量在盐胁迫24 h为对照组的1.8倍,48 h达到最高,为对照组5倍。分析表明SNP位点与相对根长、相对株高及K+、Na+含量都存在一定程度的相关性。
The study aims to find new salt-tolerant genes and breed new salt-tolerant varieties. 23 wheat varieties were used as materials to clone and sequence polymorphisms of TaPLD-2 gene, and TaPLD-2 gene expression patterns in response to salt stress was studied. The results showed that the full-length coding frame of TaPLD-2 gene was 1301 bp, located on the 2 DS chromosome, and the protein was a hydrophilic protein and located in cytoplasm. The protein had no transmembrane region and belonged to the soluble NAD(P)(H)oxidoreductase family. The TaPLD-2 gene was induced to up-regulated expression by salt stress, and the expression level was 1.8 times that of the control group at 24 h after salt stress, and reached the highest at 48 h,which was 5 times that of the control group. The analysis result showed that the SNP loci had some relevance to relative root length, relative plant height, and K+and Na+contents of salt-treated plants.
作者
宋少帅
吴凡
刘会文
贺小彦
穆平
Song Shaoshuai;Wu Fan;Liu Huiwen;He Xiaoyan;Mu Ping(College of Agronomy,Qingdao Agricultural University,Qingdao Shandong 266109)
出处
《中国农学通报》
2020年第21期98-103,共6页
Chinese Agricultural Science Bulletin
基金
山东省现代农业产业体系小麦创新团队“小麦遗传育种岗位”(SDAIT-01-05)
山东省农业良种工程项目“高效小麦分子设计育种技术体系的建立与应用”(2016LZGC-023)
关键词
小麦
基因多态性
转录组分析
盐胁迫
生物信息学分析
wheat
gene polymorphism
transcriptome analysis
salt stress
bioinformatics analysis
作者简介
第一作者简介:宋少帅,男,1992 年出生,山东茌平人,在读研究生,主要从事小麦遗传育种研究。通信地址:266000 山东省青岛市城阳区长城路700号青岛农业大学,Tel:18663974113,E-mail:1280318639@qq.com。;通讯作者:穆平,男,1971 年出生,山东博兴人,教授,研究生导师,博士,主要从事小麦遗传育种研究。通信地址:266000 山东省青岛市城阳区长城路700 号青岛农业大学,Tel:17561724586,E-mail:muping@qau.edu.cn。
