摘要
综合分析了龙虾科(Palinuridae)13个物种线粒体基因组的全序列,发现其线粒体基因组的长度为15470~16105 bp,A+T含量为62.63%~67.11%。K_(a)/K_(s)分析表明,龙虾科中的10个龙虾属(Panulirus)物种线粒体13个蛋白质编码基因(protein-codinggenes,PCGs)的K_(a)/K_(s)<<1,显示出很强的纯化选择;在差异位点的分析中,发现nd5、nd4、rrnL基因的差异位点比例较高,是理想的分子标记,可用于分析龙虾类不同群体之间的遗传多样性;密码子偏好性分析表明,被编码的氨基酸偏好性是相似的。同时,本研究采用ML(maximumlikelihood)和BI(Bayesian inference)方法构建系统发育树,结果显示,塔斯马尼亚龙虾属(Sagmariasus)物种最先开始分化,而龙虾属单独聚为一支,且与脊龙虾属(Linuparus)/游龙虾属(Puerulus)互为姊妹关系。贝叶斯分子钟估算结果显示,龙虾科物种可能起源于三叠纪,随后进一步分化为具有现代表征的龙虾种类。本研究旨在为快速鉴定龙虾科生物提供可靠的分子标记,并为分析龙虾科物种遗传多样性提供理论依据。
This study comprehensively analyzed the complete sequence of the mitochondrial genomes of 13 species in Palinuridae.The results showed that the length of the mitochondrial genome is 15470 to 16105 bp,and the A+T content is 62.63%to 67.11%.Analysis of the ratio of the number of non-synonymous substitutions per nonsynonymous site(K_(a))to the number of synonymous substitutions per synonymous site(K_(s))showed that the K_(a)/K_(s)ratio of 13 protein-coding genes(PCGs)in the mitochondria of ten Panulirus species in Palinuridae were much lower than one;this demonstrated strong purification options.In the analysis of different sites,we found that the nd5,nd4,and rrnL genes were ideal molecular markers.Codon usage analysis showed that the encoded amino acid preferences were similar.This study also adopted more systematic and comprehensive maximum likelihood(ML)and Bayesian inference(BI)methods to construct a phylogenetic tree.The results showed that the Sagmariasus first began to differentiate,and Panulirus clustered into one branch joining the Linuparus/Puerulus as sisters.The Bayesian molecular clock estimation results showed that species in Palinuridae may have originated in the Triassic,and then further differentiated into lobster species with modern characteristics.This study provides reliable molecular markers for the rapid identification of lobsters,and provides a theoretical basis to analyze the genetic diversity of lobsters.
作者
朱雷宇
朱志煌
朱陇强
林琪
ZHU Leiyu;ZHU Zhihuang;ZHU Longqiang;LIN Qi(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;Fisheries Research Institute of Fujian Province,Xiamen 361013,China;Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province,Xiamen 361013,China)
出处
《中国水产科学》
CAS
CSCD
北大核心
2022年第4期525-534,共10页
Journal of Fishery Sciences of China
基金
福建省海洋与渔业结构调整专项(2020HYJG01,2020HYJG08)
作者简介
朱雷宇(1998-),男,硕士研究生,研究方向为虾类分子系统学.E-mail:zhuleiyu1998@163.com;通信作者:林琪,研究员,研究方向为水产养殖.E-mail:xmqlin@sina.com
