摘要
目的:通过对开花促进因子AGL24基因在菘蓝不同生长时期不同部位的表达量分析,初步探索AGL24在菘蓝花发育进程中的分子调控作用。方法:以安徽亳州菘蓝居群营养生长后期的叶为试验材料提取总RNA,利用RT-PCR技术同源克隆菘蓝AGL24(IiAGL24)基因,并进行生物信息学分析。采用实时荧光定量PCR(qRT-PCR)技术分析AGL24在菘蓝不同时期不同器官中的表达情况。结果:IiAGL24基因完整编码区(CDS区)长648 bp,编码215个氨基酸。进化分析表明IiAGL24基因与十字花科植物萝卜的AGL24序列进化关系最近,同源性高达93.21%。IiAGL24在菘蓝各时期的茎、叶以及初花期的根中表达显著,且随植株进入生殖生长阶段,其表达量增加迅速。但IiAGL24在菘蓝的花、花蕾和果实中表达不显著。结论:AGL24基因在菘蓝的不同时期不同部位表达存在差异性或特异性,其广泛表达于植物的根、茎、叶和花序中,编码典型的MADS域蛋白,具有较高的研究价值。
Objective:To preliminarily explore the molecular regulation of AGL24 in the development of the Isatis indigotica flower by analyzing the expression of promoting flowering factor AGL24 gene of Isatis indigotica during different growth periods and different parts.Methods:Total RNA was extracted from leaves of Isatis indigotica in late vegetative growth period in Bozhou,Anhui province,RT-PCR was used to clone IiAGL24 gene in Isatis indigotica and bioinformatics analysis was performed.The expression of AGL24 in different organs of Isatis indigotica with different periods was analyzed by real time quantitative PCR(qRT-PCR).Results:The complete coding region(CDS region)of IiAGL24 gene was obtained,which was 648 bp and could encode 215 amino acids.The evolutionary analysis showed that IiAGL24 gene had the closest relationship with the Raphanus sativus,in which the homology was 93.21%.IiAGL24 was significantly expressed in the stems,leaves and roots of initial flowering period of Isatis indigotica in different periods and as the plant entered the reproductive growth stage,its expression increased rapidly.However,it was not significantly expressed in the flowers,buds and fruits of Isatis indigotica.Conclusion:AGL24 gene has different expression or specificity in different parts of Isatis indigotica in different periods.It is widely expressed in roots,stems,leaves and inflorescences of plants and can encode typical MADS domain proteins.It has high research significance.
作者
陈梦颖
魏莲
张慧博
崔英静
屈仁军
唐晓清
杨杰
CHEN Meng-ying;WEI Lian;ZHANG Hui-bo;CUI Ying-jing;QU Ren-jun;TANG Xiao-qing;YANG Jie(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Institute of Food Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China)
出处
《中药材》
CAS
北大核心
2019年第12期2775-2781,共7页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(31171486)
国家级大学生创新训练项目(20181037022)
中央高校基本科研业务费(Y0201900048)
2018年中医药公共卫生服务补助专项“全国中药资源普查项目”(财社[2018]43号)
关键词
菘蓝
AGL24基因
组织特异性
表达分析
Isatis indigotica Fort.
AGL24 gene
Tissue specificity
Expression analysis
作者简介
陈梦颖(1998-),女,在读本科生,专业方向:药用植物栽培和质量控制,Tel:025-84395150,E-mail:14416109@njau.edu.cn。;通讯作者:唐晓清,Tel:025-84395150,E-mail:xqtang@njau.edu.cn。
