摘要
目的通过观察针刺“曲池”“足三里”对盐敏感正常高值血压大鼠肾水通道蛋白2(Aquaporin2,AQP2)通路表达的影响,从而探讨针刺降低舒张压的作用机制。方法SPF级8周龄盐抵抗大鼠(DR)8只作为空白组,SPF级8周龄盐敏感大鼠(DS)24只,随机分为模型组、针刺组、和非穴位组,每组8只。大鼠适应性喂养1周后,各组改用8%高盐饲料喂养4周,进行模型评价,对模型评价成功的大鼠,改普通饲料喂养并同时针刺治疗4周。针刺组取“足三里”“曲池”穴施针,1次/d,6次/周,治疗4周。非穴位组针刺鼠尾距尾根部0.5 cm处,治疗方法同针刺组。治疗后测量大鼠血压值;ELISA法检测血清精氨酸加压素(AVP)含量;免疫组化法检测大鼠肾脏AQP2蛋白表达;RT-PCR检测大鼠肾脏精氨酸加压素2受体(V2R)、环磷酸腺苷(cAMP)、蛋白激酶A(PKA)、AQP2 mRNA表达。结果治疗后,与空白组比较,模型组血压升高(P<0.05),血AVP含量明显增多(P<0.05),肾AQP2蛋白表达明显上调(P<0.05),肾V2R、cAMP、PKA、AQP2mRNA表达均明显上调(P<0.05);与模型组比较,针刺组大鼠舒张压下降(P<0.05),血AVP含量明显降低(P<0.05),肾AQP2蛋白表达降低(P<0.05),肾V2R、cAMP、PKA、AQP2mRNA表达明显下调(P<0.05);与针刺组比较,非穴位组血压明显升高(P<0.05),血AVP含量明显增多(P<0.05),肾AQP2蛋白表达明显上调(P<0.05),肾V2R、cAMP、PKA、AQP2mRNA表达均明显上调(P<0.05)。结论针刺“曲池”“足三里”穴可通过下调肾AQP2的表达降低舒张压,其机制可能与抑制V2R/cAMP/PKA信号传导通路实现。
Objective To observe the effects of acupuncture at“Quchi(LI11)”and“Zusanli(ST36)”on the expression of aquaporin2(AQP2)in salt-sensitive rats with high normal blood pressure so as to explore the mechanism of acupuncture reducing diastolic blood pressure.Methods Eight SPF 8-week-old salt-resistant rats(DR)were used as the normal group,and 24 SPF 8-week-old salt-sensitive rats(DS)were randomly divided into the model group,the acupuncture group and the non-acupoint group,8 cases in each group.After the rats were adaptively fed for 1 week,each group was fed with 8%high-salt feed for 4 weeks,and the model was evaluated.After the model was successfully evaluated,the rats were fed with ordinary feed and were treated for 4 weeks.In the acupuncture group,acupuncture was given at“Quchi(LI11)”and“Zusanli(ST36)”,1 time/d,6 times/week,for 4 weeks.The non-acupoint group was given acupuncture at the rats’tail 0.5 cm away from the tail root,and the treatment method was the same as that of the acupuncture group.After treatment,the blood pressure of rats was measured.The serum AVP content was detected by ELISA.The AQP2 protein expression in the rats kidney was detected by immunohistochemical method.The expressions of V2 R,cAMP,PKA and AQP2 mRNA in the rats kidney were detected by RT-PCR.Results After treatment,compared with that of the normal group,the blood pressure of the model group was increased(P<0.05),the AVP content in the serum was significantly increased(P<0.05)and the expression of AQP2 protein in the kidney was significantly increased(P<0.05).The expressions of V2 R,cAMP,PKA and AQP2 mRNA in the kidney were all significantly up-regulated(P<0.05).Compared with that of the model group,the diastolic blood pressure of the acupuncture group was decreased(P<0.05),and the AVP content in the serum was significantly reduced(P<0.05).The AQP2 protein expression in the kidney was decreased(P<0.05).The expressions of V2 R,cAMP,PKA and AQP2 mRNA were significantly down-regulated(P<0.05).Compared with that of the acupuncture group,the blood pressure in the non-acupoint group was significantly increased(P<0.05).The AVP content in the serum was significantly increased(P<0.05),the AQP2 protein expression in the kidney was significantly up-regulated(P<0.05).The expressions of V2 R,cAMP,PKA and AQP2 mRNA in the kidney were significantly up-regulated(P<0.05).Conclusion Acupuncture at“Quchi(LI11)”and“Zusanli(ST36)”can reduce the diastolic blood pressure by down-regulating the expression of renal AQP2,and the mechanism may be related to inhibiting the realization of V2 R/cAMP/PKA signal transduction pathway.
作者
魏肖禹
曲怡
张立德
周鸿飞
WEI Xiaoyu;QU Yi;ZHANG Lide;ZHOU Hongfei(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,Liaoning,China;Liaoning University of Traditional Chinese Medicine,Ministry of Education Key Laboratory of Visceral Phenomenon Theory and Application in Traditional Chinese Medicine,Shenyang 110847,Liaoning,China)
出处
《中华中医药学刊》
CAS
北大核心
2022年第5期122-126,266,共6页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金(81302880)
辽宁省科技厅中央引导地方科技发展专项(201841601)
关键词
舒张压
针刺
水通道蛋白2
体液调节
精氨酸加压素
diastolic blood pressure
acupuncture
aquaporin 2
fluid regulation
arginine vasopressin
作者简介
魏肖禹(1993-),女,辽宁辽阳人,博士研究生,研究方向:针刺对高血压病治疗机制;通讯作者:周鸿飞(1964-),男,辽宁沈阳人,教授,博士研究生导师,硕士,研究方向:神经系统疾病针灸治疗。E-mail:hf-zhou0817@163.com。
