摘要
目的:建立HPLC波长切换法时测定健胃消炎颗粒中党参炔苷、大黄酸、大黄素、大黄酚、木香烃内酯、去氢木香内酯和芍药苷的含量。方法:以L9(34)正交试验优化提取条件,采用Agilent Extend C18色谱柱(250 mm×4.6 mm,5μm),以0.1%磷酸溶液(A)-乙腈(B)为流动相,梯度洗脱流速为1.0 ml·min-1,检测波长为230 nm(芍药苷、大黄素、大黄酸、大黄酚、木香烃内酯、去氢木香内酯)、270 nm(党参炔苷),柱温为30℃进样量为20μl。结果:党参炔苷、大黄酸、大黄素、大黄酚、木香烃内酯、去氢木香内酯和芍药苷检测质量浓度的线性范围分别为4.37~34.97,0.63~5.05,2.29~18.31,0.41~3.24,1.28~10.23,0.75~6.02,3.34~26.75μg·ml-1(r为0.9995~0.9999);平均加样回收率分别为99.7%,98.4%,99.1%,98.2%,99.2%,100.9%100.2%(RSD<2.0%,n=6)。结论:本研究建立的方法可用于健胃消炎颗粒的多组分同时测定。
Objective:To develop a method for the simultaneous determination of lobetyolin,rhein,emodin,chrysophanol,costunolide,dehydrocostuslactone and paeoniflorin in Jianwei Xiaoyan granules by HPLC with wavelength switching.Methods:L9(34)orthogonal design was carried out in order to optimize the extraction method.The analysis were carried out on a Agilent Extend C18 column(250 mmx4.6 mm,5μm)with the mobile phase consisting of 0.1%phosphoric acid(A)-methanol(B)in a gradient mode at a flow rate of 1.0 ml·min-1.The detection wavelength was set at 230 nm for rhein,emodin,chrysophanol,costunolide,dehydrocostuslactone and paeoniflorin,and 270 nm for lobetyolin.The column temperature was set at 30℃and the injection volume was 20μl.Results:The linear range was 4.37-34.97μg·ml-1 for lobetyolin,0.63-5.05μg·ml-1 for rhein,2.29-18.31μg·ml-1 for emodin,0.41-3.24μg·ml-1 for chrysophanol,1.28-10.23μg·ml-1 for costunolide,0.75-6.02μg·ml-1 for dehydrocostuslactone,and 3.34-26.75μg·ml-1for paeoniflorin(r=0.9995-0.9999).The average recovery was 99.7%,98.4%,99.1%,98.2%,99.2%,100.9%and 100.2%(RSD<2.0%,n=6).Conclusion:The method can be used to determine the multi-constituents simultaneously in Jianwei Xiaoyan granules.
作者
赵永斌
徐多麒
Zhao Yongbin;Xu Duoqi(Tianjin Dean Judicial Identification Center,Tianjin 300381,China;Chinese University of Political Science and Law)
出处
《中国药师》
CAS
2020年第3期581-585,共5页
China Pharmacist
作者简介
通讯作者:赵永斌,Tel:13920890567,E-mail:zhaoyb01@dazd.cn
