摘要
目的:观察单纯疱疹病毒性脑炎(HSE)患者脑脊液中MMP-9与MCP-1的浓度变化并探讨其意义。方法:选择HSE患者30例(HSE组),非HSE患者28例为对照组,应用ELISA法检测血清和脑脊液中MMP-9与MCP-1浓度。患者脑脊液以及加入MMP-9抑制剂SB-3CT(10μmol/L)的脑脊液分别作用于海马脑片后,TUNEL染色检测神经细胞凋亡。结果:HSE组脑脊液中MMP-9和MCP-1含量均显著高于对照组(P<0.01)。并且MMP-9与MCP-1的表达水平与脑脊液中白细胞及蛋白含量均呈正相关。抑制脑脊液中MMP-9表达可以减少神经细胞的凋亡。结论:脑脊液中MMP-9与MCP-1参与了HSE的病理生理过程,可以作为HSE的临床诊断与干预指标。
Objective:To investigate MMP-9 and MCP-1 levels in cerebrospinal fluid of patients with herpes simplex encephalitis, and to explore the clinical relationship. Method:Serum and cerebrospinal fluid MMP-9 and MCP-1 concentrations in serum and cerebrospinal fluid were detected by enzyme-linked immunosorbent assay in 30 cases with herpes simplex encephalitis(HSE group), and were compared to those in 28 cases with no HSE for control. After the patients’ cerebrospinal fluid and those added with MMP-9 inhibitor SB-3 CT(10 μmol/L) were applied to the hippocampal slices. The apoptosis of neurons was detected by TUNEL staining. Result:The CSF levels of MMP-9 and MCP-1 in HSE patients were obviously higher than those in the control group(P<0.01), and both of the expressions of MMP-9 and MCP-1 were positively correlated with the cell and protein levels in CSF(P<0.01). Inhibition of MMP-9 expression can reduce neuronal apoptosis. Conclusion:MMP-9 and MCP-1 in cerebrospinal fluid participated in the pathophysiological process of HSE, and may be used as potential markers for clinical diagnosis of HSE.
作者
周瑜
曾艳平
罗瑛
翁超
卢祖能
ZHOU Yu;ZENG Yanping;LUO Ying;WENG Chao;LU Zuneng(Department of Neurology,Renmin Hospital of Wuhan University,Wuhan,430060,China)
出处
《临床急诊杂志》
CAS
2020年第3期188-191,共4页
Journal of Clinical Emergency
基金
国家自然科学基金青年科学基金项目(No:81701196).
作者简介
通信作者:周瑜,E-mail:zhouyu99131@126.com
